Thongsongkaew Chutema, Cherisilp Benjamas, Billateh Asma, Maneechote Wageeporn, Srinuanpan Sirasit
Program of Biotechnology, Center of Excellence in Innovative Biotechnology for Sustainable Utilization of Bioresources, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
Center of Excellence of Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, Muang Chiang Mai, Chiang Mai 50200, Thailand.
J Microbiol Biotechnol. 2025 May 15;35:e2501042. doi: 10.4014/jmb.2501.01042.
This study aimed to valorize shrimp pond sludge through sequential bioprocesses using hydrolytic enzyme cocktails produced by bacterial consortia, and photosynthetic bacteria. The production of enzyme cocktails by a co-culture of protease-, amylase-, and lipase-producing bacteria (PAL) was performed in a 5-L stirred tank bioreactor using a low-cost medium. The crude enzyme cocktails were concentrated and used to treat shrimp pond sludge. The addition of enzyme cocktails at 2.0 U/ml based on protease activity led to a reduction of total suspended solids by 40.1% and an increase in soluble chemical oxygen demand (COD) by 3 folds. The solubilized nutrients from shrimp pond sludge in liquid fraction were used as a sole nutrient source to cultivate a newly isolated photosynthetic bacteria (PSB) identified as . This PSB was able to grow and achieve a high biomass of 1.30 ± 0.28 g/l and produce value-added bioproducts including aminolevulinic acid (11.77 ± 0.55 μM), carotenoids (166.84 ± 0.03 mg/g dry cell weight), and bacteriochlorophylls (771.47 ± 0.17 mg/g dry cell weight). These results highlight the potential use of enzyme cocktails produced by the co-culture of hydrolytic bacteria to facilitate the biovalorization of aquaculture sludge by PSB and may also greatly contribute to biovalorization of other similar aquaculture wastes into valuable bioproducts.
本研究旨在通过使用细菌联合体产生的水解酶混合物和光合细菌的顺序生物过程，实现虾塘污泥的增值利用。在5升搅拌罐生物反应器中，使用低成本培养基，通过共培养产蛋白酶、淀粉酶和脂肪酶的细菌（PAL）来生产酶混合物。将粗酶混合物浓缩后用于处理虾塘污泥。基于蛋白酶活性以2.0 U/ml添加酶混合物，使总悬浮固体减少了40.1%，可溶性化学需氧量（COD）增加了3倍。虾塘污泥液体部分中溶解的养分用作唯一营养源，用于培养新分离的光合细菌（PSB），该光合细菌被鉴定为 。这种PSB能够生长并达到1.30±0.28 g/l的高生物量，并产生包括氨基乙酰丙酸（11.77±0.55 μM）、类胡萝卜素（166.84±0.03 mg/g干细胞重量）和细菌叶绿素（771.47±0.17 mg/g干细胞重量）在内的增值生物产品。这些结果突出了水解细菌共培养产生的酶混合物在促进PSB对水产养殖污泥进行生物增值利用方面的潜在用途，也可能极大地有助于将其他类似的水产养殖废物生物转化为有价值的生物产品。
