大规模对大鼠海马体中的内源性单细胞进行蛋白质异构体分析。
Proteoform profiling of endogenous single cells from rat hippocampus at scale.
作者信息
Su Pei, Hollas Michael A R, Pla Indira, Rubakhin Stanislav, Butun Fatma Ayaloglu, Greer Joseph B, Early Bryan P, Fellers Ryan T, Caldwell Michael A, Sweedler Jonathan V, Kafader Jared O, Kelleher Neil L
机构信息
Department of Chemistry, Northwestern University, Evanston, IL, USA.
Proteomics Center of Excellence, Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.
出版信息
Nat Biotechnol. 2025 May 15. doi: 10.1038/s41587-025-02669-x.
We perform intact proteoform profiling of 10,809 endogenous single cells from the rat hippocampus using single-cell proteoform imaging mass spectrometry (scPiMS). scPiMS directly extracts whole proteins and demonstrates high throughput for MS-based single-cell proteomics compared with existing approaches. We develop an informatics workflow dedicated to this datatype and use it to assign neurons, astrocytes or microglia cell types according to their proteoform signatures.
我们使用单细胞蛋白异构体成像质谱法（scPiMS）对来自大鼠海马体的10,809个内源性单细胞进行完整蛋白异构体分析。与现有方法相比，scPiMS可直接提取完整蛋白质，并在基于质谱的单细胞蛋白质组学中展现出高通量的特性。我们开发了一种专门针对此数据类型的信息学工作流程，并利用它根据蛋白质异构体特征来区分神经元、星形胶质细胞或小胶质细胞类型。
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