Department of Genome Sciences, University of Washington, Seattle, WA, USA.
International Centre for Cancer Vaccine Science, University of Gdańsk, Gdańsk, Poland.
Nat Methods. 2023 Mar;20(3):339-346. doi: 10.1038/s41592-023-01802-5.
Mammalian cells have about 30,000-fold more protein molecules than mRNA molecules, which has major implications in the development of proteomics technologies. We review strategies that have been helpful for counting billions of protein molecules by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and suggest that these strategies can benefit single-molecule methods, especially in mitigating the challenges of the wide dynamic range of the proteome.
哺乳动物细胞中的蛋白质分子数量比 mRNA 分子多约 30,000 倍，这对蛋白质组学技术的发展具有重要意义。我们综述了通过液相色谱-串联质谱 (LC-MS/MS) 计数数十亿个蛋白质分子的有用策略，并提出这些策略可以使单分子方法受益，特别是在缓解蛋白质组宽动态范围的挑战方面。
