CRISPR/Cas12a 在病原体快速检测中的应用。
Application of CRISPR/Cas12a in the rapid detection of pathogens.
机构信息
Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China; National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou 510642, China; National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
出版信息
Clin Chim Acta. 2023 Aug 1;548:117520. doi: 10.1016/j.cca.2023.117520. Epub 2023 Aug 16.
The combination of clustered regularly interspaced short palindromic repeats (CRISPR) and its associated Cas protein is an effective gene-editing instrument. Among them, the CRISPR-Cas12a system forms a DNA-cleavage-capable complex with crRNA and exerts its trans-cleavage activity by recognising the PAM site on the target pathogen's gene. After amplifying the pathogenic gene, display materials such as fluorescent probes are added to the detection system, along with the advantages of rapid detection and high sensitivity of the CRISPR system, so that pathogenic bacteria can be diagnosed with greater speed and precision. This article reviews the mechanism of CRISPR-Cas12a in rapid detection, as well as its progress in the rapid detection of pathogenic bacteria in conjunction with various molecular biology techniques, in order to provide a foundation for the future development of a more effective detection platform.
成簇规律间隔短回文重复序列（CRISPR）及其相关的 Cas 蛋白是一种有效的基因编辑工具。其中，CRISPR-Cas12a 系统与 crRNA 形成具有 DNA 切割能力的复合物，并通过识别靶病原体基因上的 PAM 位点发挥其转录切割活性。在扩增致病基因后，将荧光探针等显示材料添加到检测系统中，同时结合 CRISPR 系统快速检测和高灵敏度的优势，从而可以更快、更精准地诊断致病菌。本文综述了 CRISPR-Cas12a 在快速检测中的作用机制，以及其与各种分子生物学技术相结合在快速检测致病菌方面的进展，以期为未来开发更有效的检测平台提供基础。
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