Oyama Taigo, Imashiro Chikahiro, Kurashina Yuta, Ando Keita, Takemura Kenjiro
School of Science for Open and Environmental Systems Graduate School of Science and Technology Keio University Yokohama Japan.
Department of Mechanical Engineering Keio University Yokohama Japan.
Eng Life Sci. 2025 May 15;25(5):e70011. doi: 10.1002/elsc.70011. eCollection 2025 May.
Sonochemistry has become increasingly important in bioengineering research, and many in vitro and in vivo bioapplications have been developed. Cytotoxicity is always a concern in its implementation. For in vivo treatments and studies, mechanical index (MI) is known to ensure biocompatibility, and even in vitro MI has been used. Because cell characteristics and acoustic phenomena differ in vitro and in vivo, we questioned using MI in vitro. The in vitro cytotoxicity of ultrasound exposure should be investigated to support the development of cutting-edge sonochemistry. In this study, a system for irradiating cultured cells with 1-2 MHz-range ultrasound was developed to demonstrate the invalidity of employing MI alone in vitro. The results showed that cell damage is defined by the MI, ultrasound frequency, and exposure time, which are new indices for quantifying cell damage. Furthermore, cavitation and acoustic streaming are shown to be the main scientific factors that injure cells.
声化学在生物工程研究中变得越来越重要，并且已经开发了许多体外和体内生物应用。细胞毒性在其应用中一直是一个关注点。对于体内治疗和研究，已知机械指数（MI）可确保生物相容性，甚至体外也使用了MI。由于体外和体内的细胞特性及声学现象不同，我们对在体外使用MI提出质疑。应研究超声暴露的体外细胞毒性，以支持前沿声化学的发展。在本研究中，开发了一种用于用1-2MHz范围的超声照射培养细胞的系统，以证明仅在体外使用MI是无效的。结果表明，细胞损伤由MI、超声频率和暴露时间决定，这些是量化细胞损伤的新指标。此外，空化和声流被证明是损伤细胞的主要科学因素。
