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用于检测未浓缩脑脊液中寡克隆带的高电渗琼脂糖电泳和硝酸纤维素固定法

High electroendosmotic agarose electrophoresis and nitrocellulose immobilisation for the detection of oligoclonal bands in unconcentrated cerebrospinal fluid.

作者信息

Keir G, Chowhan M R, Thompson E J

出版信息

Ann Clin Biochem. 1985 Jul;22 ( Pt 4):381-6. doi: 10.1177/000456328502200409.

DOI:10.1177/000456328502200409
PMID:4037665
Abstract

A sensitive (1 microL of unconcentrated sample), specific (IgG Fc) and economical (10 p/test) method for the detection of oligoclonal bands in cerebrospinal fluid is presented. Using commonly available technology (agarose gel electrophoresis) and giving results within 6 h, the approach used here is a powerful tool in the investigation of immunoglobulin abnormalities in many biological fluids.

摘要

本文介绍了一种灵敏(1微升未浓缩样品)、特异(IgG Fc)且经济(每次检测10便士)的检测脑脊液中寡克隆带的方法。该方法采用常用技术(琼脂糖凝胶电泳),6小时内即可得出结果,是研究多种生物体液中免疫球蛋白异常情况的有力工具。

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