Keir G, Walker R W, Johnson M H, Thompson E J
Clin Chim Acta. 1982 May 20;121(2):231-6. doi: 10.1016/0009-8981(82)90063-8.
A method is presented which enables the study of cerebrospinal fluid proteins in minimal volumes of unconcentrated fluid. The method involves agarose electrophoresis followed by transfer of the proteins to a nitrocellulose membrane and then demonstration of their distribution using a double-antibody immunoperoxidase technique. The method is used here for the study of immunoglobulin G and its subgroups, and clearly shows that the oligoclonal pattern observed in multiple sclerosis is not restricted to any one subgroup.
本文介绍了一种方法，该方法能够在最小体积的未浓缩脑脊液中研究脑脊液蛋白质。该方法包括琼脂糖电泳，随后将蛋白质转移至硝酸纤维素膜上，然后使用双抗体免疫过氧化物酶技术展示其分布情况。本文使用该方法研究免疫球蛋白G及其亚组，并清楚地表明在多发性硬化症中观察到的寡克隆模式并不局限于任何一个亚组。
