Morozov Viktor V, Balakireva Anastasia V, Perfilov Maxim M, Chepurnykh Tatyana V, Yampolsky Ilia V, Sarkisyan Karen S, Mishin Alexander S
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia.
Planta LLC, Moscow 121205, Russia.
ACS Synth Biol. 2025 Jun 20;14(6):2375-2379. doi: 10.1021/acssynbio.4c00802. Epub 2025 May 16.
Engineering of orthogonal systems functional across diverse hosts can benefit from employing universal regulatory DNA elements. Here, we screened a number of composite promoters in plant, fungal, and bacterial hosts, identifying variants that drive strong constitutive expression in , , , and , or only in the eukaryotic subset of these organisms. These promoters can be used in universal vectors to co-optimize for different hosts in directed evolution, engineering of biosynthetic pathways, or other biotechnological tasks that require host switching.
构建能在多种宿主中发挥功能的正交系统可受益于使用通用调控DNA元件。在此，我们在植物、真菌和细菌宿主中筛选了多个复合启动子，鉴定出能在植物、真菌、细菌以及仅在这些生物的真核亚群中驱动强组成型表达的变体。这些启动子可用于通用载体，以便在定向进化、生物合成途径工程或其他需要宿主切换的生物技术任务中针对不同宿主进行共同优化。
