Sheikholeslami Samin, Amiri-Andebili Marziyeh, Baghaei Amir, Ahmadian-Attari Mohammad Mahdi
Evidence-based Phytotherapy and Complementary Medicine Research Center, Alborz University of Medical Sciences, Karaj, Iran; Department of Pharmacognosy, Faculty of Pharmacy, Alborz University of Medical Sciences, Karaj, Iran.
Department of Toxicology and Pharmacology, Faculty of Pharmacy, Alborz University of Medical Sciences, Karaj, Iran.
Ann Pharm Fr. 2025 May 14. doi: 10.1016/j.pharma.2025.05.004.
This short communication presents an analytical approach to evaluate the synergistic anticonvulsant effects of an herbal combination containing hydroalcoholic extracts of Origanum vulgare (oregano), Matricaria chamomilla (chamomile), and Lavandula angustifolia (lavender).
The study aimed to apply linear dose-response modeling to seizure score data to predict the expected outcomes of combination therapy and quantify synergy by comparing predicted and observed effects.
The herbal extracts were obtained through maceration with 70% ethanol. The animal study was performed using 2 different doses of each herb, involving 36 rats in 6 groups. Building on previous in vivo findings, the study utilized linear dose-response modeling to analyze the effects of the combination therapy on seizure scores and to determine the synergistic performance at combination doses of 17mg/kg, 34mg/kg, and 68mg/kg.
The results highlighted the synergistic performance of the herbal combination, particularly at the dose of 34mg/kg, demonstrating the pharmacodynamic advantages of combination therapy and confirming the enhanced antiseizure effect of the herbal combination.
This study confirms the synergistic anticonvulsant effects of the herbal combination and demonstrates its potential as a new supplementary treatment for seizures via a non-invasive intranasal administration method.
本简短通讯介绍了一种分析方法，用于评估一种草药组合的协同抗惊厥作用，该组合包含牛至（奥勒冈草）、母菊（洋甘菊）和薰衣草的水醇提取物。
本研究旨在将线性剂量反应模型应用于癫痫发作评分数据，以预测联合治疗的预期结果，并通过比较预测效果和观察效果来量化协同作用。
通过用70%乙醇浸渍获得草药提取物。动物研究使用每种草药的2种不同剂量，涉及6组36只大鼠。基于先前的体内研究结果，该研究利用线性剂量反应模型分析联合治疗对癫痫发作评分的影响，并确定17mg/kg、34mg/kg和68mg/kg联合剂量下的协同性能。
结果突出了该草药组合的协同性能，特别是在34mg/kg剂量下，证明了联合治疗的药效学优势，并证实了该草药组合增强的抗癫痫作用。
本研究证实了该草药组合的协同抗惊厥作用，并证明了其通过非侵入性鼻内给药方法作为癫痫新辅助治疗的潜力。
