内含子微小RNA介导调控的荧光素酶报告基因
The Luciferase Reporter on Intronic MicroRNA-Directed Regulation.
作者信息
Xu WeiBo, Guo Qianhuan, Zhang ShuXin, Yan Kang
机构信息
National Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, China.
College of Agriculture and Biology, Liaocheng University, Liaocheng, China.
出版信息
Methods Mol Biol. 2025;2900:203-212. doi: 10.1007/978-1-0716-4398-3_13.
The Luciferase reporter detects and characterizes genes by using Luciferin as a substrate. Intronic miRNAs are located within intron and derived from the processing of their hosting pre-mRNA. Here, we describe the Luciferase reporter system used in the detection of intron splicing events affecting the intronic miRNA processing by this method.
荧光素酶报告基因通过使用荧光素作为底物来检测和表征基因。内含子miRNA位于内含子内，由其宿主前体mRNA的加工产生。在此，我们描述了通过这种方法用于检测影响内含子miRNA加工的内含子剪接事件的荧光素酶报告基因系统。
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