抗原相关的牛冠状病毒和人冠状病毒OC43的寡核苷酸指纹图谱
Oligonucleotide fingerprints of antigenically related bovine coronavirus and human coronavirus OC43.
作者信息
Lapps W, Brian D A
出版信息
Arch Virol. 1985;86(1-2):101-8. doi: 10.1007/BF01314116.
Abstract
Virion RNAs from the bovine enteric coronavirus and the human respiratory coronavirus OC43 were compared by one dimensional gel electrophoresis and by oligonucleotide fingerprinting. For each virus, approximately 55 per cent of the RNA migrated as a 6.8 Md species, 10 per cent as a 0.68 Md species, and 15 per cent as heterogeneous small molecular weight RNA. A sequence homology of greater than 96 per cent was observed between the 6.8 Md species from the two viruses. The 0.68 Md RNA is apparently an intravirion, subgenomic, polyadenylated molecule based on RNAse studies, oligo (dT)-cellulose chromatography, and hybridization to a cDNA clone of the 3' terminal 1.19 Kb region of the bovine coronavirus genome.
摘要
通过一维凝胶电泳和寡核苷酸指纹图谱对牛肠道冠状病毒和人呼吸道冠状病毒OC43的病毒粒子RNA进行了比较。对于每种病毒，约55%的RNA以6.8 Md的条带迁移，10%以0.68 Md的条带迁移，15%以异质的小分子RNA形式迁移。两种病毒的6.8 Md条带之间观察到大于96%的序列同源性。基于核糖核酸酶研究、寡聚（dT）-纤维素色谱分析以及与牛冠状病毒基因组3'末端1.19 Kb区域的cDNA克隆杂交，0.68 Md RNA显然是一种病毒内的亚基因组多聚腺苷酸化分子。
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