Chen Xiaoning, Shen Bing, Lin Weijie, Xiong Ziqi, Yang Bohao, Luo Hanxi, Zong Zhiwei, Chen Jie, Bahabayi Ayibaota, Liu Chen
Department of Clinical Laboratory, Peking University People's Hospital, 11 Xizhimen South Street, Beijing, 100044, China.
Institute of Medical Technology, Peking University Health Science Center, Beijing, China.
Immunol Res. 2025 May 17;73(1):83. doi: 10.1007/s12026-025-09637-2.
This study aims to analyze CD27 expression in various subsets of CD4+ T cells in peripheral blood, explore the functional characteristics of the CD27+ subsets in regulatory T cells (Tregs) and CD4+ T cells, and assess their immunological alterations in newly diagnosed systemic lupus erythematosus (SLE) patients.
Peripheral blood was collected from 34 newly diagnosed, untreated SLE patients and 22 healthy controls. Flow cytometry was used to analyze CD27 expression on T cell subsets, comparing the functional markers between CD27+ and CD27- subsets. CD27 expression on Tregs and total CD4+ T cells in SLE patients and healthy controls were compared. ROC curves were constructed, and areas under the curve (AUCs) was calculated to evaluate the diagnostic value of CD27-related T cell subsets for SLE.
The proportion of Tregs in the peripheral blood of SLE patients was increased, and CD27 expression was higher in Tregs than in CD4+ T cells in healthy individuals. CD27+ CD4+ T cells exhibited higher CD45RA and lower CD226 expression. CD27+ Tregs showed higher Helios and TIGIT expression and lower CD226 expression. CD27+ cell proportions in both CD4+ T cells and Tregs were significantly reduced in SLE patients. The AUC for CD27-related T cell subsets in diagnosing newly diagnosed SLE ranged from 0.6238 to 0.86.
CD27 CD4 T cells show reduced activation features, while CD27 Tregs exhibit enhanced regulatory potential. Their decreased proportions in SLE patients suggest early immune dysregulation.
本研究旨在分析外周血中CD4+ T细胞各亚群中CD27的表达情况，探究调节性T细胞（Tregs）和CD4+ T细胞中CD27+亚群的功能特征，并评估新诊断的系统性红斑狼疮（SLE）患者中这些细胞的免疫变化。
收集34例新诊断、未治疗的SLE患者和22例健康对照者的外周血。采用流式细胞术分析T细胞亚群上CD27的表达，比较CD27+和CD27-亚群之间的功能标志物。比较SLE患者和健康对照者Tregs及总CD4+ T细胞上CD27的表达。构建ROC曲线并计算曲线下面积（AUC），以评估CD27相关T细胞亚群对SLE的诊断价值。
SLE患者外周血中Tregs比例升高，且健康个体中Tregs的CD27表达高于CD4+ T细胞。CD27+ CD4+ T细胞表现出较高的CD45RA表达和较低的CD226表达。CD27+ Tregs表现出较高的Helios和TIGIT表达以及较低的CD226表达。SLE患者中CD4+ T细胞和Tregs的CD27+细胞比例均显著降低。CD27相关T细胞亚群诊断新诊断SLE的AUC范围为0.6238至0.86。
CD27 CD4 T细胞显示出激活特征降低，而CD27 Tregs表现出增强的调节潜能。它们在SLE患者中的比例降低提示早期免疫失调。
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