Pollock J A, Lipson E D, Sullivan D T
Biochem Genet. 1985 Jun;23(5-6):379-90. doi: 10.1007/BF00499081.
Using two-dimensional gel electrophoresis, we have analyzed proteins from a plasma membrane-enriched fraction from Phycomyces sporangiophores. Specifically, we have compared gels for night-blind mutants and a wild-type strain to find proteins involved in the early steps of the sensory transduction chain for phototropism. In the gels for a mutant affected in the gene madA, a protein spot [51 kilodaltons (kdal) and pI 6.35] appears that is absent from the wild-type and the other mad mutants. Mutants affect in either of two madB alleles lack a protein spot (57 kdal and pI 6.6) that is present in the wild-type and all other mad strains; this spot probably represents the madB gene product. In some madC mutants, two spots (59 kdal, pI 6.5, with a covalently linked flavin; and 50 kdal, pI 6.4) are absent; however, in other madC strains, one or both of these spots are present. These four protein spots that are altered in madA, madB, and madC mutants may represent component of the photoreceptor complex responsible for phototropism in Phycomyces.
利用二维凝胶电泳技术，我们分析了来自毛霉属孢子囊柄富含质膜部分的蛋白质。具体而言，我们比较了夜盲突变体和野生型菌株的凝胶，以寻找参与向光性感觉转导链早期步骤的蛋白质。在受madA基因突变影响的突变体的凝胶中，出现了一个蛋白质斑点（51千道尔顿（kdal），等电点6.35），而野生型和其他mad突变体中没有该斑点。受两个madB等位基因中任何一个影响的突变体缺少一个蛋白质斑点（57 kdal，等电点6.6），该斑点存在于野生型和所有其他mad菌株中；这个斑点可能代表madB基因产物。在一些madC突变体中，两个斑点（59 kdal，等电点6.5，带有共价连接的黄素；和50 kdal，等电点6.4）缺失；然而，在其他madC菌株中，这些斑点中的一个或两个都存在。在madA、madB和madC突变体中发生改变的这四个蛋白质斑点可能代表毛霉属中负责向光性的光受体复合物的组成部分。
