壁内胚层样F9细胞中层粘连蛋白亚基的翻译后组装和糖基化
Post-translational assembly and glycosylation of laminin subunits in parietal endoderm-like F9 cells.
作者信息
Morita A, Sugimoto E, Kitagawa Y
出版信息
Biochem J. 1985 Jul 1;229(1):259-64. doi: 10.1042/bj2290259.
Abstract
Non-reducing and reducing sodium dodecyl sulphate/polyacrylamide-gel electrophoresis of laminin synthesized in parietal endoderm-like F9 cells demonstrated that only AB1B2 complex goes through intracellular traffic for oligosaccharide side-chain processing and secretion. Glycosylation was not necessary for subunit assembly. Assembly was suggested to proceed through B1B2 to AB1B2. Among the pools of monomer subunits, the B2 pool was smallest.
摘要
对在类壁内胚层F9细胞中合成的层粘连蛋白进行非还原和还原十二烷基硫酸钠/聚丙烯酰胺凝胶电泳表明，只有AB1B2复合物经历细胞内运输以进行寡糖侧链加工和分泌。糖基化对于亚基组装并非必需。推测组装过程是通过B1B2到AB1B2进行的。在单体亚基库中，B2库最小。
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