Laurie G W, Leblond C P, Martin G R, Silver M H
J Histochem Cytochem. 1982 Oct;30(10):991-8. doi: 10.1177/30.10.6752265.
Reichert's membrane and the endodermal cells of the parietal yolk sac were examined for the presence of laminin antigenicity using anti-laminin antibodies and the peroxidase-antiperoxidase sequence. Immunostaining was observed through the full width of Reichert's membrane and within endodermal cells. In these cells immunostaining was observed in rough endoplasmic reticulum (rER) cisternae and Golgi apparatus. The Golgi staining could occur in any saccule, but predominated in components interpreted as the last saccule of the stack, the GERL element, and associated prosecretory granules. The secretory granules found in the ectoplasm were also immunostained. Finally, multivesicular bodies showed some staining. The immunostaining of Reichert's membrane indicates the presence of laminin itself, while that of rER cisternae and the Golgi apparatus is attributed to laminin precursors. Presumably the biosynthesis of laminin occurs along the usual protein pathway, that is, from rER through Golgi saccules and the GERL element to secretory granules, which release their content into Reichert's membrane. The laminin immunostaining of Reichert's membrane and endodermal cells is similar to that of type IV collagen. It is, therefore, likely that the two substances are processed and secreted simultaneously.
使用抗层粘连蛋白抗体和过氧化物酶-抗过氧化物酶序列，检测赖歇特膜和壁层卵黄囊的内胚层细胞中是否存在层粘连蛋白抗原性。在赖歇特膜的整个宽度以及内胚层细胞内均观察到免疫染色。在这些细胞中，在粗面内质网（rER）池和高尔基体中观察到免疫染色。高尔基体染色可发生在任何囊泡中，但在被解释为堆叠的最后一个囊泡、GERL元件以及相关的分泌前颗粒的成分中占主导。在外质中发现的分泌颗粒也被免疫染色。最后，多囊泡体显示出一些染色。赖歇特膜的免疫染色表明层粘连蛋白本身的存在，而rER池和高尔基体装置的免疫染色则归因于层粘连蛋白前体。推测层粘连蛋白的生物合成沿着通常的蛋白质途径进行，即从rER通过高尔基体囊泡和GERL元件到分泌颗粒，分泌颗粒将其内容物释放到赖歇特膜中。赖歇特膜和内胚层细胞的层粘连蛋白免疫染色与IV型胶原相似。因此，这两种物质可能同时进行加工和分泌。
