Docherty J C, Brown S B
Biochem J. 1984 Sep 1;222(2):401-6. doi: 10.1042/bj2220401.
Human haemoglobin was prepared containing [14C]haem in either the alpha- or the beta-subunits. Coupled oxidation of such hybrid haemoglobins with ascorbate and O2 showed that the biliverdin produced by the alpha-subunits contained approx. 55% alpha-isomer and 45% beta-isomer, whereas that produced by the beta-subunits contained approx. 75% alpha-isomer and 25% beta-isomer. Coupled oxidation of isolated alpha- and beta-subunits gave approx. 70% alpha-isomer, 30% beta-isomer and 78% alpha-isomer, 22% beta-isomer respectively. These results are consistent with calculations of differences in the haem environment in the two subunit types.
制备了α亚基或β亚基含有[¹⁴C]血红素的人血红蛋白。这种杂合血红蛋白与抗坏血酸和氧气的偶联氧化表明，α亚基产生的胆绿素约含55%的α异构体和45%的β异构体，而β亚基产生的胆绿素约含75%的α异构体和25%的β异构体。分离的α亚基和β亚基的偶联氧化分别产生约70%的α异构体、30%的β异构体和78%的α异构体、22%的β异构体。这些结果与两种亚基类型中血红素环境差异的计算结果一致。
