Sunamoto J, Baba Y, Iwamoto K, Kondo H
Biochim Biophys Acta. 1985 Jan 9;833(1):144-50.
Autoxidation of egg phosphatidylcholines and polyunsaturated fatty acids, arachidonic and linoleic acids, has been investigated in homogeneous and liposomal membrane systems. In order to monitor the very initial stage of the radical chain mechanism of the autoxidation, an improved method using 1,1-diphenyl-2-picrylhydrazyl radical was newly implemented and used with the regular thiobarbituric acid test to determine the peroxides of polyunsaturated fatty acids. Autoxidation of polyunsaturated fatty acids or lipids was significantly enhanced in liposomal bilayers compared to that in a bulk homogeneous solution. In liposomal bilayers, the reaction could be controlled by membrane fluidity, which was confirmed by the fluorescence polarization technique using 1,6-diphenylhexatriene and dansylhexadecylamine. Even vitamin E esters such as the acetate and the pivalate could depress effectively the autoxidation of egg phosphatidylcholines in bilayer systems, which supported Lucy's proposal (Diplock, A.T. and Lucy, J.A. (1973) FEBS Lett. 29, 205-210) about the importance of the side-chain of vitamin E as an antioxidant.
已在均相和脂质体膜系统中研究了鸡蛋磷脂酰胆碱以及多不饱和脂肪酸（花生四烯酸和亚油酸）的自氧化作用。为了监测自氧化自由基链机制的最初阶段，新采用了一种使用1,1 - 二苯基 - 2 - 苦基肼自由基的改进方法，并与常规的硫代巴比妥酸试验一起用于测定多不饱和脂肪酸的过氧化物。与本体均相溶液相比，脂质体双层中多不饱和脂肪酸或脂质的自氧化作用显著增强。在脂质体双层中，该反应可由膜流动性控制，这通过使用1,6 - 二苯基己三烯和丹磺酰十六胺的荧光偏振技术得到证实。甚至维生素E酯（如乙酸酯和新戊酸酯）也能有效抑制双层系统中鸡蛋磷脂酰胆碱的自氧化作用，这支持了露西关于维生素E侧链作为抗氧化剂重要性的提议（迪普洛克，A.T.和露西，J.A.（1973年）《欧洲生物化学学会联合会快报》29，205 - 210）。
