Ball R Y, Carpenter K L, Enright J H, Hartley S L, Mitchinson M J
Br J Exp Pathol. 1987 Jun;68(3):427-38.
Murine resident peritoneal macrophages were maintained in cell culture in a medium containing 10% lipoprotein-deficient fetal calf serum to which various artificial lipoproteins (lipid-bovine serum albumin complexes) had been added. Ceroid accumulated in cells exposed to artificial lipoproteins containing cholesteryl esters or acylglycerols possessing polyunsaturated fatty acid residues, but not in cells exposed to lipoproteins containing less readily oxidized lipids. Oxidation of cholesteryl linoleate before its incorporation into artificial lipoprotein accelerated ceroid production. Incorporation of free radical scavengers into cholesteryl linoleate-containing artificial lipoproteins impaired ceroid formation. The results are discussed in terms of the mechanisms by which the ceroid might have been produced and its significance for human atherogenesis.
将小鼠腹腔常驻巨噬细胞置于含有10%脂蛋白缺陷型胎牛血清的细胞培养基中培养，该培养基中已添加了各种人工脂蛋白（脂质 - 牛血清白蛋白复合物）。在暴露于含有胆固醇酯或具有多不饱和脂肪酸残基的酰基甘油的人工脂蛋白的细胞中会积累蜡样质，但在暴露于含有较不易氧化脂质的脂蛋白的细胞中则不会。亚油酸胆固醇酯在掺入人工脂蛋白之前的氧化会加速蜡样质的产生。将自由基清除剂掺入含亚油酸胆固醇酯的人工脂蛋白中会损害蜡样质的形成。本文从蜡样质可能产生的机制及其对人类动脉粥样硬化形成的意义方面对结果进行了讨论。
