• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

传染性造血坏死病毒mRNA种类的特征揭示了一种非病毒粒子弹状病毒蛋白。

Characterization of infectious hematopoietic necrosis virus mRNA species reveals a nonvirion rhabdovirus protein.

作者信息

Kurath G, Leong J C

出版信息

J Virol. 1985 Feb;53(2):462-8. doi: 10.1128/JVI.53.2.462-468.1985.

DOI:10.1128/JVI.53.2.462-468.1985
PMID:4038520
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC254658/
Abstract

The genome RNA and six mRNA species of infectious hematopoietic necrosis virus were analyzed by denaturing gel electrophoresis. The following molecular weights were determined: genome RNA, 3.7 X 10(6); mRNA 1, 2.26 X 10(6); mRNA 2, 5.63 X 10(5); mRNA 3, 4.84 X 10(5); mRNA 4 (containing two different mRNA species), 3.00 X 10(5); and mRNA 5, 1.95 X 10(5). Densitometer analyses of gels were used to calculate the molar ratios of the intracellular mRNA species: mRNA 1, 0.02; mRNA 2, 0.49; mRNA 3, 1.0; mRNA 4, 2.52; and mRNA 5, 0.41. Hybrid selection studies determined the mRNA coding assignments as follows: mRNA 1 encodes the viral polymerase, L; mRNA 2 encodes the glycoprotein, G; mRNA 3 encodes the nucleocapsid protein, N; mRNA 4 is composed of two comigrating mRNA species which encode the matrix proteins, M1 and M2; and mRNA 5 encodes a previously unrecognized viral protein which is induced in infected cells but is not present in mature virions. This nonvirion protein has been designated the NV protein.

摘要

采用变性凝胶电泳分析了传染性造血坏死病毒的基因组RNA和六种mRNA。测定了以下分子量：基因组RNA为3.7×10⁶；mRNA 1为2.26×10⁶；mRNA 2为5.63×10⁵；mRNA 3为4.84×10⁵；mRNA 4（包含两种不同的mRNA）为3.00×10⁵；mRNA 5为1.95×10⁵。利用凝胶密度计分析计算细胞内mRNA的摩尔比：mRNA 1为0.02；mRNA 2为0.49；mRNA 3为1.0；mRNA 4为2.52；mRNA 5为0.41。杂交筛选研究确定了mRNA的编码分配如下：mRNA 1编码病毒聚合酶L；mRNA 2编码糖蛋白G；mRNA 3编码核衣壳蛋白N；mRNA 4由两种共迁移的mRNA组成，编码基质蛋白M1和M2；mRNA 5编码一种以前未识别的病毒蛋白，该蛋白在感染细胞中被诱导产生，但不存在于成熟病毒粒子中。这种非病毒粒子蛋白被命名为NV蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/205b/254658/618de9ee9b29/jvirol00125-0132-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/205b/254658/35f0258d3ed9/jvirol00125-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/205b/254658/dea267bac2dc/jvirol00125-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/205b/254658/618de9ee9b29/jvirol00125-0132-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/205b/254658/35f0258d3ed9/jvirol00125-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/205b/254658/dea267bac2dc/jvirol00125-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/205b/254658/618de9ee9b29/jvirol00125-0132-a.jpg

相似文献

1
Characterization of infectious hematopoietic necrosis virus mRNA species reveals a nonvirion rhabdovirus protein.传染性造血坏死病毒mRNA种类的特征揭示了一种非病毒粒子弹状病毒蛋白。
J Virol. 1985 Feb;53(2):462-8. doi: 10.1128/JVI.53.2.462-468.1985.
2
Molecular cloning of the six mRNA species of infectious hematopoietic necrosis virus, a fish rhabdovirus, and gene order determination by R-loop mapping.传染性造血器官坏死病毒（一种鱼类弹状病毒）六种mRNA的分子克隆及通过R环作图法确定基因顺序
J Virol. 1985 Feb;53(2):469-76. doi: 10.1128/JVI.53.2.469-476.1985.
3
Distant strains of the fish rhabdovirus VHSV maintain a sixth functional cistron which codes for a nonstructural protein of unknown function.鱼类弹状病毒水疱性口炎病毒（VHSV）的远亲毒株保留了第六个功能顺反子，该顺反子编码一种功能未知的非结构蛋白。
Virology. 1995 Oct 1;212(2):741-5. doi: 10.1006/viro.1995.1534.
4
Identification and analysis of Ebola virus messenger RNA.
Virology. 1987 Apr;157(2):414-20. doi: 10.1016/0042-6822(87)90283-2.
5
The NV genes of fish rhabdoviruses: development of RNase protection assays for rapid assessment of genetic variation.鱼类弹状病毒的NV基因：用于快速评估遗传变异的核糖核酸酶保护分析方法的开发
Vet Res. 1995;26(5-6):477-85.
6
Identification of a tenth mRNA of respiratory syncytial virus and assignment of polypeptides to the 10 viral genes.呼吸道合胞病毒第十种mRNA的鉴定及多肽与10个病毒基因的对应关系
J Virol. 1984 Feb;49(2):572-8. doi: 10.1128/JVI.49.2.572-578.1984.
7
Coding assignments of the five smaller mRNAs of Newcastle disease virus.新城疫病毒五种较小mRNA的编码分配
J Virol. 1982 Sep;43(3):1024-31. doi: 10.1128/JVI.43.3.1024-1031.1982.
8
Nucleotide sequence of a cDNA clone carrying the glycoprotein gene of infectious hematopoietic necrosis virus, a fish rhabdovirus.携带传染性造血器官坏死病毒（一种鱼类弹状病毒）糖蛋白基因的cDNA克隆的核苷酸序列。
J Virol. 1987 May;61(5):1342-9. doi: 10.1128/JVI.61.5.1342-1349.1987.
9
Structure analysis of the rice yellow stunt rhabdovirus glycoprotein gene and its mRNA.
Arch Virol. 1998;143(12):2453-9. doi: 10.1007/s007050050475.
10
Human parainfluenza virus type 3: messenger RNAs, polypeptide coding assignments, intergenic sequences, and genetic map.人3型副流感病毒：信使核糖核酸、多肽编码分配、基因间序列和遗传图谱。
J Virol. 1986 Sep;59(3):646-54. doi: 10.1128/JVI.59.3.646-654.1986.

引用本文的文献

1
Plasticity, Paralogy, and Pseudogenization: Rhabdoviruses of Freshwater Mussels Elucidate Mechanisms of Viral Genome Diversification and the Evolution of the Finfish-Infecting Rhabdoviral Genera.可塑性、旁系同源和假基因化：淡水贻贝类弹状病毒阐明了病毒基因组多样化的机制以及感染硬骨鱼类的弹状病毒属的进化。
J Virol. 2023 May 31;97(5):e0019623. doi: 10.1128/jvi.00196-23. Epub 2023 May 8.
2
The efficacy of new oral vaccine feeds against in rainbow trout.新型口服疫苗饲料对虹鳟鱼的功效。
Fish Shellfish Immunol Rep. 2023 Jan 4;4:100082. doi: 10.1016/j.fsirep.2023.100082. eCollection 2023 Dec.
3
Revised Taxonomy of Rhabdoviruses Infecting Fish and Marine Mammals.

本文引用的文献

1
Rabies virus-induced RNA synthesis in BHK21 cells.狂犬病病毒诱导的BHK21细胞中的RNA合成。
J Gen Virol. 1980 Jul;49(1):181-95. doi: 10.1099/0022-1317-49-1-181.
2
The structural proteins of rabies virus and evidence for their synthesis from separate monocistronic RNA species.狂犬病病毒的结构蛋白及其由单独的单顺反子RNA物种合成的证据。
J Gen Virol. 1980 Jul;49(1):161-80. doi: 10.1099/0022-1317-49-1-161.
3
In vitro translation of rabies virus messenger RNAs.狂犬病病毒信使核糖核酸的体外翻译
感染鱼类和海洋哺乳动物的弹状病毒修订分类法。
Animals (Basel). 2022 May 26;12(11):1363. doi: 10.3390/ani12111363.
4
Molecular Epidemiology of Novirhabdoviruses Emerging in Iranian Trout Farms.伊朗鳟鱼养殖场新出现的诺维氏弹状病毒的分子流行病学
Viruses. 2021 Mar 10;13(3):448. doi: 10.3390/v13030448.
5
Transcriptome profiling in head kidney of rainbow trout (Oncorhynchus mykiss) after infection with the low-virulent Nagano genotype of infectious hematopoietic necrosis virus.虹鳟鱼（Oncorhynchus mykiss）感染低毒力长野基因型传染性造血器官坏死病毒后头肾转录组谱分析。
Arch Virol. 2021 Apr;166(4):1057-1070. doi: 10.1007/s00705-021-04980-9. Epub 2021 Feb 2.
6
Transcriptome analysis based on RNA-seq of common innate immune responses of flounder cells to IHNV, VHSV, and HIRRV.基于 RNA-seq 的牙鲆细胞对 IHNV、VHSV 和 HIRRV 共同先天免疫反应的转录组分析。
PLoS One. 2020 Sep 28;15(9):e0239925. doi: 10.1371/journal.pone.0239925. eCollection 2020.
7
Molecular and functional insights into a novel teleost malectin from big-belly seahorse Hippocampus abdominalis.分子和功能研究揭示了来自大腹海马 Hippocampus abdominalis 的新型硬脂酰基转移酶。
Fish Shellfish Immunol. 2020 Apr;99:483-494. doi: 10.1016/j.fsi.2020.02.044. Epub 2020 Feb 20.
8
Differential Immune Transcriptome and Modulated Signalling Pathways in Rainbow Trout Infected with Viral Haemorrhagic Septicaemia Virus (VHSV) and Its Derivative Non-Virion (NV) Gene Deleted.感染病毒性出血性败血症病毒（VHSV）及其缺失非病毒体（NV）基因的衍生物的虹鳟鱼的差异免疫转录组和信号通路调控
Vaccines (Basel). 2020 Jan 30;8(1):58. doi: 10.3390/vaccines8010058.
9
NV Proteins of Fish Novirhabdovirus Recruit Cellular PPM1Bb Protein Phosphatase and Antagonize RIG-I-Mediated IFN Induction.鱼类诺如病毒 NV 蛋白招募细胞 PPM1Bb 蛋白磷酸酶并拮抗 RIG-I 介导的 IFN 诱导。
Sci Rep. 2017 Mar 9;7:44025. doi: 10.1038/srep44025.
10
The family Rhabdoviridae: mono- and bipartite negative-sense RNA viruses with diverse genome organization and common evolutionary origins.弹状病毒科：具有多样基因组结构和共同进化起源的单分体和双分体负链RNA病毒。
Virus Res. 2017 Jan 2;227:158-170. doi: 10.1016/j.virusres.2016.10.010. Epub 2016 Oct 20.
Virology. 1980 Jun;103(2):517-21. doi: 10.1016/0042-6822(80)90211-1.
4
The viral diseases of fish: a review through 1978. Part 1: Diseases of proven viral etiology.鱼类病毒性疾病：截至1978年的综述。第1部分：已证实病毒病因的疾病。
Crit Rev Microbiol. 1980;7(4):287-363. doi: 10.3109/10408418009077984.
5
Nucleotide sequences of the mRNA's encoding the vesicular stomatitis virus N and NS proteins.编码水疱性口炎病毒N蛋白和NS蛋白的信使核糖核酸的核苷酸序列。
J Virol. 1981 Aug;39(2):529-35. doi: 10.1128/JVI.39.2.529-535.1981.
6
Nucleotide sequences of the mRNA's encoding the vesicular stomatitis virus G and M proteins determined from cDNA clones containing the complete coding regions.从包含完整编码区的cDNA克隆中确定的编码水疱性口炎病毒G蛋白和M蛋白的mRNA的核苷酸序列。
J Virol. 1981 Aug;39(2):519-28. doi: 10.1128/JVI.39.2.519-528.1981.
7
Localized attenuation and discontinuous synthesis during vesicular stomatitis virus transcription.水泡性口炎病毒转录过程中的局部衰减和不连续合成。
Cell. 1981 Feb;23(2):477-84. doi: 10.1016/0092-8674(81)90143-4.
8
Calcium protects DNase I from proteinase K: a new method for the removal of contaminating RNase from DNase I.钙可保护脱氧核糖核酸酶I免受蛋白酶K的作用：一种从脱氧核糖核酸酶I中去除污染性核糖核酸酶的新方法。
Anal Biochem. 1980 Sep 1;107(1):260-4. doi: 10.1016/0003-2697(80)90519-9.
9
Complete intergenic and flanking gene sequences from the genome of vesicular stomatitis virus.水疱性口炎病毒基因组的完整基因间隔区及侧翼基因序列。
Cell. 1980 Feb;19(2):415-21. doi: 10.1016/0092-8674(80)90515-2.
10
Hybridization of denatured RNA transferred or dotted nitrocellulose paper.转移或点样在硝酸纤维素膜上的变性RNA的杂交。
Methods Enzymol. 1983;100:255-66. doi: 10.1016/0076-6879(83)00060-9.