Farmand Sahand, Ahmed Emaan, Zawar Hadisa Azizi, Thuret Sandrine
Department of Basic and Clinical Neuroscience, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London, United Kingdom.
Aging Brain. 2025 Apr 30;7:100138. doi: 10.1016/j.nbas.2025.100138. eCollection 2025.
Selenium has been shown to be a key regulatory element in the health, survival and proliferation of neural stem and progenitor cells, with various studies underlining its anti-aging properties. However, most of this knowledge is derived from rodent models, leaving its effects on human hippocampal progenitor cells unclear. In this study, we utilized a human hippocampal progenitor cell (HPC) line to examine the effects of varying concentrations of sodium selenite, an inorganic form of selenium (0 µM, 0.1 µM, 0.23 µM, 0.5 µM, and 1.0 µM), on the proliferation, apoptosis, and progenitor integrity of these cells. To do this, HPCs were exposed to these concentrations for 48 h, followed by immunocytochemistry to quantify, cell number (DAPI-positive cells), proliferation (KI67-positve cells), apoptosis (CC3-positve cells), and progenitor integrity (SOX2- and Nestin-positive cells). While our results indicated no significant effects of selenium concentrations on proliferation or apoptosis, we demonstrated that absence of selenium (0 μM) in the culture media significantly reduced both cell number and percentage of Nestin-positive cells, but only when compared to the condition with the highest selenium concentration (1.0 μM). Our findings underscore the role of selenium in regulating the survival and integrity of human HPCs. Lastly, we emphasize the need for further research to uncover the mechanisms underlying these observed changes.
硒已被证明是神经干细胞和祖细胞健康、存活及增殖的关键调节元素，多项研究强调了其抗衰老特性。然而，这些知识大多来源于啮齿动物模型，其对人类海马祖细胞的影响尚不清楚。在本研究中，我们利用一种人类海马祖细胞（HPC）系，来检测不同浓度的亚硒酸钠（一种无机形式的硒，浓度分别为0 μM、0.1 μM、0.23 μM、0.5 μM和1.0 μM）对这些细胞增殖、凋亡及祖细胞完整性的影响。为此，将HPCs暴露于这些浓度下48小时，随后进行免疫细胞化学分析，以量化细胞数量（DAPI阳性细胞）、增殖（KI67阳性细胞）、凋亡（CC3阳性细胞）及祖细胞完整性（SOX2和巢蛋白阳性细胞）。虽然我们的结果表明硒浓度对增殖或凋亡没有显著影响，但我们证明，培养基中缺乏硒（0 μM）会显著降低细胞数量和巢蛋白阳性细胞的百分比，但这仅与硒浓度最高（1.0 μM）的条件相比时才成立。我们的研究结果强调了硒在调节人类HPCs存活和完整性方面的作用。最后，我们强调需要进一步研究以揭示这些观察到的变化背后的机制。
