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利用高分辨率质谱对初乳棒进行代谢组学分析以鉴定功能化合物

Metabolomic profiling of colostrum bar for identification of functional compounds using high resolution mass spectroscopy.

作者信息

Tiwari Shivam, Poonia Amrita, Rao Vasundhara

机构信息

Department of Dairy Science and Food Technology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005 India.

出版信息

J Food Sci Technol. 2025 Jun;62(6):1123-1140. doi: 10.1007/s13197-024-06097-8. Epub 2024 Sep 25.

Abstract

In this era of hectic lifestyle no one have ample time to take care of their health and generally people miss some nutrients daily. This study has shown that, how to use low price ingredients such as colostrum (unutilized part of dairy farm), flax seed, moringa leaves powder, fresh dates, and gram flour to formulate functional colostrum bar to tackle nutrients deficiency. Optimization process was performed on the basis of sensory attributes, and further functional compounds profiling was done by High Resolution Accurate Mass Spectroscopy, which identified about 980 compounds in total, 60 of which could be positively identified. Phenolic substances, including chlorogenic acid as well as caffeic acid, which have anti-inflammatory and antioxidant effects, were prominent. The optimized sample (E4) also contained flavonoids with different classes which includes, N-acetyl-α-D-glucosamine (Flavonoid glycosides), Aspalathin, Quercetin 3-O-rhamnoside (flavonoid-3-o-glycosides), glucosiduronic acid, Quercetin (Flavanols), Kaempferol (Flavonols), Luteolin (flavones), Kaempferol 3-O-glucoside-7-O-rhamnoside, Rutin (flavonoid glycoside), Apigenin 6-C-alpha-L-arabinopyranosyl-8-C-beta-D-xylopyranoside (flavanone) and Myricetin 3-O-β-D-Galactopyranoside (glycosyloxyflavone). By increasing the utilization of colostrum, possibly improving the socio-economic status of farmers, and providing a health-promoting product that fits with consumer trends towards more sustainable and healthier food options.

摘要

在这个生活节奏忙碌的时代,没有人有充足的时间照顾自己的健康,人们通常每天都会错过一些营养物质。这项研究表明,如何使用初乳(奶牛场未利用的部分)、亚麻籽、辣木叶粉、鲜枣和鹰嘴豆粉等低价成分来配制功能性初乳棒,以解决营养缺乏问题。基于感官属性进行了优化过程,并通过高分辨率精确质谱对进一步的功能化合物进行了分析,总共鉴定出约980种化合物,其中60种可以得到明确鉴定。具有抗炎和抗氧化作用的酚类物质,包括绿原酸和咖啡酸,最为突出。优化后的样品(E4)还含有不同种类的黄酮类化合物,包括N-乙酰-α-D-葡萄糖胺(黄酮糖苷)、阿萨伊拉辛、槲皮素3-O-鼠李糖苷(黄酮-3-o-糖苷)、葡萄糖醛酸、槲皮素(黄烷醇)、山奈酚(黄酮醇)、木犀草素(黄酮)、山奈酚3-O-葡萄糖苷-7-O-鼠李糖苷、芦丁(黄酮糖苷)、芹菜素6-C-α-L-阿拉伯吡喃糖基-8-C-β-D-木吡喃糖苷(黄烷酮)和杨梅素3-O-β-D-吡喃半乳糖苷(糖基氧基黄酮)。通过提高初乳的利用率,可能改善农民的社会经济地位,并提供一种符合消费者对更可持续和更健康食品选择趋势的健康促进产品。

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