特异性抗红细胞灶形成作为衡量NZB小鼠自身抗体分泌细胞的指标。
Specific anti-erythrocyte focus formation as a measure of autoantibody secreting cells in NZB mice.
作者信息
Moore J, Calkins C
出版信息
Clin Exp Immunol. 1985 Jan;59(1):153-60.
Abstract
Using a modification of the antibody forming cell (AFC) focus assay, it is possible to quantitate the spontaneous anti-erythrocyte autoantibody response of New Zealand Black (NZB) mice at the level of the autoantibody secreting cell. This complement independent assay is specific for an antigen(s) present on unmodified mouse erythrocytes. A comparison with the direct Coombs' test showed that the focus assay detected autoantibody responses earlier and demonstrated a wide range of AFC levels in responding mice. The focus assay provides a method for evaluating autoimmunity at the cellular level and for investigating the activities of cells responding to autoantigens.
摘要
采用抗体形成细胞（AFC）灶性分析法的一种改良方法，能够在自身抗体分泌细胞水平对新西兰黑（NZB）小鼠的自发性抗红细胞自身抗体反应进行定量。这种不依赖补体的分析方法对未修饰小鼠红细胞上存在的一种或多种抗原具有特异性。与直接库姆斯试验的比较表明，灶性分析法能更早地检测到自身抗体反应，并在有反应的小鼠中显示出广泛的AFC水平范围。灶性分析法提供了一种在细胞水平评估自身免疫以及研究对自身抗原作出反应的细胞活性的方法。
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The secretion of antibody by isolated lymph node cells.分离的淋巴结细胞分泌抗体。
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Organ-specific IgM autoantibodies to liver, heart and brain in man: generalized occurrence and possible functional significance in normal individuals, and studies in patients with multiple sclerosis.人体内针对肝脏、心脏和大脑的器官特异性IgM自身抗体：在正常个体中的普遍存在及可能的功能意义，以及对多发性硬化症患者的研究。
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In vitro generation of cells producing antierythrocyte autoantibodies in normal mice.正常小鼠体内产生抗红细胞自身抗体细胞的体外生成
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Formation of antigen specific foci as a complement independent assay for individual antibody-secreting cells.形成抗原特异性灶作为一种针对单个抗体分泌细胞的非补体依赖性检测方法。
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Radioimmunoassay for immunoglobulin G autoantibody on the surface of mouse erythrocytes.小鼠红细胞表面免疫球蛋白G自身抗体的放射免疫测定
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Natural antibodies against tubulin, actin myoglobin, thyroglobulin, fetuin, albumin and transferrin are present in normal human sera, and monoclonal immunoglobulins from multiple myeloma and Waldenström's macroglobulinemia may express similar antibody specificities.正常人血清中存在针对微管蛋白、肌动蛋白、肌红蛋白、甲状腺球蛋白、胎球蛋白、白蛋白和转铁蛋白的天然抗体，多发性骨髓瘤和瓦尔登斯特伦巨球蛋白血症患者的单克隆免疫球蛋白可能表达相似的抗体特异性。
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