Buyukyildirim Tugsen, Ocal Ozdamar Fahriye, Baysal Furtana Gokcen, Gok Hasya Nazli, Ozek Temel, Orhan Ilkay Erdogan, Senol Deniz F Sezer, Duman Hayri
Department of Pharmacognosy, Faculty of Pharmacy, Selcuk University, Konya, Türkiye.
Department of Biology, Faculty of Science, Gazi University, Ankara, Türkiye.
Chem Biodivers. 2025 Sep;22(9):e202500328. doi: 10.1002/cbdv.202500328. Epub 2025 May 19.
The genus Satureja (Lamiaceae) includes many aromatic species with known antimicrobial, antioxidant, anti-inflammatory, and anticancer properties. Satureja hasturkii H.Duman & Dirmenci is a newly described species from Türkiye (2023), and its biological potential has not been explored yet. In this study, dichloromethane (DCM), ethyl acetate (EtOAc), and ethanol (EtOH) extracts were assessed for their enzyme inhibitory activity against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), elastase, collagenase, tyrosinase (TYR), and xanthine oxidase (XO), along with their antioxidant activities using DPPH, nitric oxide (NO), metal-chelation, ferric-reducing antioxidant power (FRAP), and cupric-reducing antioxidant capacity (CUPRAC) assays. The extracts showed low to moderate inhibition levels at 2 mg/mL (stock concentration). The EtOAc extract showed the most effective elastase (16.70 ± 2.08%) and collagenase (13.96 ± 3.70%) inhibition. XO inhibition was highest with EtOH (34.34 ± 0.60%), followed by EtOAc extract (30.32 ± 2.03%). DCM extract displayed the strongest metal-chelating activity (72.38 ± 1.86%). Essential oil composition analyzed by GC-MS revealed docosane, γ-terpinene, and p-cymene as major constituents. HPLC analysis of the extracts identified rosmarinic acid and luteolin-7-O-glucoside as abundant phenolics. These results highlight the species' potential as a natural source of bioactive agents for pharmaceutical and nutraceutical applications.
鼠尾草属（唇形科）包含许多具有抗菌、抗氧化、抗炎和抗癌特性的芳香植物物种。哈氏鼠尾草（Satureja hasturkii H.Duman & Dirmenci）是2023年在土耳其新描述的物种，其生物学潜力尚未得到探索。在本研究中，评估了二氯甲烷（DCM）、乙酸乙酯（EtOAc）和乙醇（EtOH）提取物对乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）、弹性蛋白酶、胶原酶、酪氨酸酶（TYR）和黄嘌呤氧化酶（XO）的酶抑制活性，以及使用DPPH、一氧化氮（NO）、金属螯合、铁还原抗氧化能力（FRAP）和铜还原抗氧化能力（CUPRAC）测定法测定的抗氧化活性。提取物在2mg/mL（储备浓度）时表现出低至中等的抑制水平。乙酸乙酯提取物对弹性蛋白酶（16.70±2.08%）和胶原酶（13.96±3.70%）的抑制作用最为有效。乙醇对XO的抑制作用最高（34.34±0.60%），其次是乙酸乙酯提取物（30.32±2.03%）。二氯甲烷提取物表现出最强的金属螯合活性（72.38±1.86%）。通过气相色谱-质谱联用（GC-MS）分析的精油成分显示，二十二烷、γ-萜品烯和对伞花烃为主要成分。提取物的高效液相色谱（HPLC）分析确定迷迭香酸和木犀草素-7-O-葡萄糖苷为丰富的酚类物质。这些结果突出了该物种作为药物和营养保健品应用中生物活性剂天然来源的潜力。
