Dracopoli N C, Rettig W J, Albino A P, Esposito D, Archidiacono N, Rocchi M, Siniscalco M, Old L J
Am J Hum Genet. 1985 Jan;37(1):199-207.
The monoclonal antibody AbO13 defines a cell-surface antigen that is expressed on most cultured human cells, but not on rodent cells. AbO13 precipitates glycoproteins of 25,000 and 30,000 mol. wt. from lysates of [3H]glucosamine-labeled human cells. Results of the serological typing of a panel of 25 rodent-human somatic cell hybrid clones show that reactivity with AbO13 segregates with the human X and Y chromosomes. The presence of either of these chromosomes is sufficient for O13 expression on the hybrid cell surface. Analysis of hybrid clones containing human X chromosomes with karyotypically defined deletions permitted the regional assignment of the X-linked gene locus controlling the expression of O13 to Xp22-pter. In addition, AbO13 is reactive with Chinese hamster-human hybrids derived from fibroblasts of a 49,XXXXX individual that contained only inactivated copies of the human X chromosome. These results suggest that the X-linked locus determining the expression of O13 is not subject to X-inactivation.
单克隆抗体AbO13可识别一种细胞表面抗原，该抗原在大多数培养的人类细胞上表达，但在啮齿动物细胞上不表达。AbO13可从[3H]葡萄糖胺标记的人类细胞裂解物中沉淀出分子量为25,000和30,000的糖蛋白。对一组25个啮齿动物 - 人类体细胞杂交克隆进行血清学分型的结果表明，与AbO13的反应性与人类X和Y染色体相关。这两条染色体中的任何一条的存在都足以使杂交细胞表面表达O13。对含有经核型定义的缺失的人类X染色体的杂交克隆进行分析，可将控制O13表达的X连锁基因座定位到Xp22 - pter区域。此外，AbO13与源自一名49,XXXXX个体成纤维细胞的中国仓鼠 - 人类杂交细胞反应，这些杂交细胞仅含有失活的人类X染色体拷贝。这些结果表明，决定O13表达的X连锁基因座不受X染色体失活的影响。
