Rettig W J, Dracopoli N C, Goetzger T A, Spengler B A, Biedler J L, Oettgen H F, Old L J
Proc Natl Acad Sci U S A. 1984 Oct;81(20):6437-41. doi: 10.1073/pnas.81.20.6437.
The expression of 13 newly defined human cell surface antigens identified by monoclonal antibodies was studied in a panel of reduced rodent-human somatic cell hybrid clones. For each antigenic system the segregation of antibody reactivity was concordant with the segregation of a specific human chromosome, permitting the chromosomal assignment of 13 gene loci determining antigen expression. The antigens can be placed in four groups on the basis of their patterns of control in the hybrid cells. (i) Presence of a single human chromosome is necessary and sufficient for antigen expression; L230 (assigned to chromosome 2), AJ425, K15 (chromosome 3), SR84 (chromosome 5), JF23, Q14 (chromosome 11), SV13 (chromosome 15), and F10 (chromosome 19). (ii) AJ2 (chromosome 10) and J143 (chromosome 17); two antigens coded for by separate human chromosomes but associated as a molecular complex on the surface of AJ2+/J143+ human cells. (iii) F8 (chromosome 19); antigen expression dependent on the growth characteristics of hybrid cells: substrate-adherent cells are F8+, whereas cells growing in suspension are F8-. (iv) AO122 and F23 (chromosome 15); antigen expression controlled by the permissive/inducing vs. nonpermissive/noninducing nature of the rodent fusion partner. Hybrids derived from both antigen-positive and antigen-negative human cells can express AO122 and F23 but only when specific rodent cell types are used for hybridization: N4TG-1 neuroblastoma and L cells, but not RAG renal carcinoma cells, permit AO122 expression, whereas RAG and L cells, but not N4TG-1 cells, permit F23 expression. The rapidly expanding list of monoclonal antibodies defining human cell surface molecules provides a range of markers to probe the genetic regulation of antigen diversity in somatic cells.
在一组经减数分裂的啮齿动物 - 人类体细胞杂交克隆中，研究了由单克隆抗体鉴定出的13种新定义的人类细胞表面抗原的表达情况。对于每个抗原系统，抗体反应性的分离与特定人类染色体的分离一致，从而确定了13个决定抗原表达的基因座的染色体定位。根据它们在杂交细胞中的控制模式，这些抗原可分为四组。（i）单个人类染色体的存在对于抗原表达是必要且充分的；L230（定位于2号染色体）、AJ425、K15（3号染色体）、SR84（5号染色体）、JF23、Q14（11号染色体）、SV13（15号染色体）和F10（19号染色体）。（ii）AJ2（10号染色体）和J143（17号染色体）；两种抗原由不同的人类染色体编码，但在AJ2 + / J143 +人类细胞表面作为分子复合物相关联。（iii）F8（19号染色体）；抗原表达取决于杂交细胞的生长特性：贴壁生长的细胞为F8 +，而悬浮生长的细胞为F8 -。（iv）AO122和F23（15号染色体）；抗原表达受啮齿动物融合伙伴的允许/诱导与非允许/非诱导性质控制。来自抗原阳性和抗原阴性人类细胞的杂交体均可表达AO122和F23，但仅当使用特定类型的啮齿动物细胞进行杂交时：N4TG - 1神经母细胞瘤和L细胞，但不是RAG肾癌细胞，允许AO122表达，而RAG和L细胞，但不是N4TG - 1细胞，允许F23表达。定义人类细胞表面分子的单克隆抗体列表迅速增加，提供了一系列标记物来探究体细胞中抗原多样性的遗传调控。
