Chen T R
Cytogenet Cell Genet. 1985;39(1):57-63. doi: 10.1159/000132103.
G-banded and C-banded prometaphase and metaphase karyotypes of the Chinese hamster Don cell line from three different laboratories were compared. All the cell lines were pseudodiploid. Each consisted of two distinct cell populations designated S1 and S2. The chromosome complements of each of these populations differed from the diploid by only one additional chromosome band on a subterminal 1p in S1 and on a median 1q in S2. The sites of these extra bands were neither constricted nor heterochromatic. All other different karyotypes, including those from seven distinct subpopulations, had basic patterns of chromosome complements that were best represented by S1 or S2, respectively the major or minor cell type of the cell line. Moreover, nearly 80% of the metaphases analyzed had a modal chromosome number of 22, and about 60% had the same chromosome composition of a cell type or subtype. Our data also suggest that Don cells were probably pseudodiploid by 1964 or before.
比较了来自三个不同实验室的中国仓鼠Don细胞系的G带和C带早中期及中期核型。所有细胞系均为假二倍体。每个细胞系都由两个不同的细胞群体组成，分别命名为S1和S2。这些群体中每个群体的染色体组与二倍体的差异仅在于S1的亚端粒1p上和S2的中着丝粒1q上各有一条额外的染色体带。这些额外带的位置既没有缢缩也不是异染色质。所有其他不同的核型，包括来自七个不同亚群体的核型，其染色体组的基本模式分别由S1或S2（分别为细胞系的主要或次要细胞类型）最佳代表。此外，所分析的近80%的中期细胞具有22条的众数染色体数，约60%具有相同的细胞类型或亚型的染色体组成。我们的数据还表明，Don细胞在1964年或之前可能就是假二倍体。
