Ray F A, Bartholdi M F, Kraemer P M, Cram L S
Cytogenet Cell Genet. 1984;38(4):257-64. doi: 10.1159/000132072.
A chromosome polymorphism was detected between two early passage euploid Chinese hamster cell strains when a fluorescence shift of the small metacentric No. 9 chromosome was resolved by flow cytometry. The characteristics of the polymorphism were studied using cultures established from ear clippings taken from 16 additional hamsters from our breeding colony. Additional variants of chromosome 9 were detected using flow cytometry, and a subset of these variants were analyzed by G- and C-banding. An increase of fluorescence recorded by flow cytometry correlated with an increase of centromeric heterochromatin. Autosomal normalization of the flow karyotype from 18 different animals indicated three distinct peak positions for chromosome 9. The results indicate that a discrete block of constitutive heterochromatin may be present in one or two extra copies within the small inbred colony of hamsters studied. To determine the inheritance patterns, hamsters with known polymorphic No. 9 chromosomes were bred. The flow karyotypes derived from the offspring of these matings provide strong evidence that chromosomal polymorphisms are inherited in Mendelian fashion.
当通过流式细胞术解析小的中着丝粒9号染色体的荧光变化时，在两个早期传代的整倍体中国仓鼠细胞系之间检测到一种染色体多态性。利用从我们繁殖群体中另外16只仓鼠的耳部剪下的组织建立的培养物，研究了这种多态性的特征。使用流式细胞术检测到9号染色体的其他变体，并通过G带和C带分析了这些变体的一个子集。流式细胞术记录的荧光增加与着丝粒异染色质的增加相关。来自18只不同动物的流式核型的常染色体标准化显示9号染色体有三个不同的峰值位置。结果表明，在所研究的仓鼠小近交群体中，可能存在一到两个额外拷贝的离散组成型异染色质块。为了确定遗传模式，对具有已知多态性9号染色体的仓鼠进行了繁殖。这些交配后代的流式核型提供了强有力的证据，证明染色体多态性是以孟德尔方式遗传的。
