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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1977 Mar 1;161(3):543-9. doi: 10.1042/bj1610543.

A stable, more highly purified, preparation of UDP-glucuronyltransferase was obtained than previously reported. 2. Enzyme activity towards o-aminophenyl and p-nitrophenyl was increased 43- and 46-fold respectively. 3. The final preparation contains only three staining polypeptide bands visible after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. 4. The only known major accompanying protein appears to be epoxide hydratase. 5. The purified enzyme activity towards o-aminophenol can still be activated 3 fold by diethylnitrosamine. 6. On evidence from purification, o-aminophenol and p-nitrophenol appear to be glucuronidated by the same enzyme protein. The possible recognition of the UDP-glucuronyltransferase enzyme is discussed.

获得了一种比之前报道的更稳定、纯度更高的UDP-葡萄糖醛酸基转移酶制剂。2. 对邻氨基苯和对硝基苯的酶活性分别提高了43倍和46倍。3. 最终制剂在十二烷基硫酸钠/聚丙烯酰胺凝胶电泳后仅含有三条可见的染色多肽带。4. 唯一已知的主要伴随蛋白似乎是环氧化物水合酶。5. 纯化后的酶对邻氨基酚的活性仍可被二乙基亚硝胺激活3倍。6. 根据纯化证据，邻氨基酚和对硝基苯酚似乎由同一种酶蛋白进行葡萄糖醛酸化。讨论了UDP-葡萄糖醛酸基转移酶可能的识别情况。

Biochem J. 1977 Mar 1;161(3):543-9. doi: 10.1042/bj1610543.

A stable, more highly purified, preparation of UDP-glucuronyltransferase was obtained than previously reported. 2. Enzyme activity towards o-aminophenyl and p-nitrophenyl was increased 43- and 46-fold respectively. 3. The final preparation contains only three staining polypeptide bands visible after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. 4. The only known major accompanying protein appears to be epoxide hydratase. 5. The purified enzyme activity towards o-aminophenol can still be activated 3 fold by diethylnitrosamine. 6. On evidence from purification, o-aminophenol and p-nitrophenol appear to be glucuronidated by the same enzyme protein. The possible recognition of the UDP-glucuronyltransferase enzyme is discussed.

获得了一种比之前报道的更稳定、纯度更高的UDP-葡萄糖醛酸基转移酶制剂。2. 对邻氨基苯和对硝基苯的酶活性分别提高了43倍和46倍。3. 最终制剂在十二烷基硫酸钠/聚丙烯酰胺凝胶电泳后仅含有三条可见的染色多肽带。4. 唯一已知的主要伴随蛋白似乎是环氧化物水合酶。5. 纯化后的酶对邻氨基酚的活性仍可被二乙基亚硝胺激活3倍。6. 根据纯化证据，邻氨基酚和对硝基苯酚似乎由同一种酶蛋白进行葡萄糖醛酸化。讨论了UDP-葡萄糖醛酸基转移酶可能的识别情况。