大鼠肝脏中针对睾酮和雌酮的UDP-葡萄糖醛酸基转移酶活性的分离与纯化。
The separation and purification of rat liver UDP-glucuronyltransferase activities towards testosterone and oestrone.
作者信息
Weatherill P J, Burchell B
出版信息
Biochem J. 1980 Aug 1;189(2):377-80. doi: 10.1042/bj1890377.
Abstract
- Reconstitution of UDP-glucuronyltransferase preparations with phosphataidylcholine liposomes facilitated the purification of testosterone UDP-glucuronyltransferase. 2. Transferase activity towards testosterone co-purifies with that towards 4-nitrophenol. 3. UDP-glucuronyltransferase activity towards oestrone was separated from that towards testosterone. 4. These results suggest that testosterone and 4-nitrophenol may be glucuronidated by a different form of UDP-glucuronyltransferase from the one glucuronidating oestrone.
摘要
- 用磷脂酰胆碱脂质体重组UDP-葡萄糖醛酸基转移酶制剂有助于睾酮UDP-葡萄糖醛酸基转移酶的纯化。2. 对睾酮的转移酶活性与对4-硝基苯酚的转移酶活性共同纯化。3. 对雌酮的UDP-葡萄糖醛酸基转移酶活性与对睾酮的UDP-葡萄糖醛酸基转移酶活性分离。4. 这些结果表明，睾酮和4-硝基苯酚可能由一种不同于使雌酮葡萄糖醛酸化的UDP-葡萄糖醛酸基转移酶形式进行葡萄糖醛酸化。
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