辅酶Q10对磷脂酶作用的影响。

The effect of coenzyme Q10 on the action of phospholipase.

作者信息

Sugiyama S, Hattori M, Nagai S, Takamura T, Ozawa T

出版信息

Arzneimittelforschung. 1985;35(1):23-5.

Abstract

Using dimyristoyl L-alpha-phosphatidylcholine as a substrate, the effect of coenzyme Q10 on phospholipid digestion by phospholipase A2 and phospholipase C was investigated. Free myristic acids released by phospholipase A2, and myristic acids of dimyristoyl glyceride released by phospholipase C were methylated and determined quantitatively by gas-chromatography. Phospholipase A2 or phospholipase C released myristic acids dose-dependently from the substrate. Coenzyme Q10 prevented dose-dependently the hydrolysis of the substrate caused by phospholipase. These results suggest that pharmacological action of coenzyme Q10 could be attributed to its protection of membrane phospholipids against the attack of phospholipases.

摘要

以二肉豆蔻酰-L-α-磷脂酰胆碱为底物，研究了辅酶Q10对磷脂酶A2和磷脂酶C催化磷脂消化的影响。磷脂酶A2释放的游离肉豆蔻酸以及磷脂酶C释放的二肉豆蔻酰甘油的肉豆蔻酸经甲基化后，采用气相色谱法定量测定。磷脂酶A2或磷脂酶C从底物中剂量依赖性地释放肉豆蔻酸。辅酶Q10剂量依赖性地抑制了磷脂酶引起的底物水解。这些结果表明，辅酶Q10的药理作用可能归因于其对膜磷脂免受磷脂酶攻击的保护作用。