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两种对云杉色卷蛾致病的杆状病毒株的N-多角体蛋白特性分析

Characterization of N-polyhedrin of two baculovirus strains pathogenic for Orgyia pseudotsugata.

作者信息

Rohrmann G F

出版信息

Biochemistry. 1977 Apr 19;16(8):1631-4. doi: 10.1021/bi00627a017.

DOI:10.1021/bi00627a017
PMID:403932
Abstract

N-polyhedrin of inclusion bodies of two nucleopolyhedrosis viruses of Orgyia pseudotsugata was characterized. Alkali-dissolved N-polyhedrin from both virus strains was of similar size and consisted of 12S molecule of 209 000 daltons. Eight subunits of approximately 26 000 daltons were found to form the 12S molecules. N-polyhedrin from both viruses showed two main antigens by immunodiffusion. The subunits appear to possess one antigen and, upon formation of the 12S molecule, a new antigen is created. Both the subunit and 12S antigens from the two virus strains were shown to be antigenically related. The 12S molecule of both viruses also appears to possess a minor antigen unique to each virus.

摘要

对云杉芽卷叶蛾两种核型多角体病毒包涵体的N-多角体蛋白进行了特性分析。两种病毒株的碱溶性N-多角体蛋白大小相似,由209000道尔顿的12S分子组成。发现由大约26000道尔顿的八个亚基形成12S分子。两种病毒的N-多角体蛋白通过免疫扩散显示出两种主要抗原。亚基似乎具有一种抗原,并且在形成12S分子时,会产生一种新的抗原。两种病毒株的亚基和12S抗原均显示出抗原相关性。两种病毒的12S分子似乎还具有每种病毒特有的一种次要抗原。

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Characterization of N-polyhedrin of two baculovirus strains pathogenic for Orgyia pseudotsugata.两种对云杉色卷蛾致病的杆状病毒株的N-多角体蛋白特性分析
Biochemistry. 1977 Apr 19;16(8):1631-4. doi: 10.1021/bi00627a017.
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