Rohrmann G F, Pearson M N, Bailey T J, Becker R R, Beaudreau G S
J Mol Evol. 1981;17(6):329-33. doi: 10.1007/BF01734354.
A phylogenetic tree for occluded baculoviruses was constructed based on the N-terminal amino acid sequence of occlusion body proteins from six baculoviruses including three lepidopteran nuclear polyhedrosis viruses (NPVs), [two unicapsid (Bombyx mori and Orgyia pseudotsugata) and one multicapsid (Orgyia pseudotsugata)]; one granulosis virus (Pieris brassicae); and NPVs from a hymenopteran (Neodiprion sertifer) and a dipteran (Tipula paludosa). Amino acid sequence data for the B. mori NPV were from a report by Serebryani et al. (1977) and that for the O. pseudotsugata NPVs were reported previously by us (Rohrmann et al. 1979). The other N-terminal amino acid sequences are presented in this paper. The phylogenetic relationships determined based on the molecular evolution of polyhedrin were also investigated by antigenic comparisons of the proteins using a solid phase radioimmune assay. The results indicate that the lepidopteran NPVs are the most closely related of the above group of viruses and are related to these viruses in the following order: N. sertifer NPV, P. brassicae granulosis virus, and T. paludosa NPV. These data, in conjunction with Baculovirus distribution and evidence concerning insect phylogeny, suggest that the Baculovirus have an ancient association with insects and may havae evolved along with them.
基于六种杆状病毒的包涵体蛋白N端氨基酸序列构建了一种被膜杆状病毒的系统发育树，这六种杆状病毒包括三种鳞翅目核型多角体病毒（NPV），[两种单粒包埋型（家蚕和黄杉毒蛾）和一种多粒包埋型（黄杉毒蛾）]；一种颗粒体病毒（粉纹夜蛾）；以及来自膜翅目（欧洲松叶蜂）和双翅目（大蚊）的NPV。家蚕NPV的氨基酸序列数据来自Serebryani等人（1977年）的一份报告，黄杉毒蛾NPV的氨基酸序列数据此前由我们报道过（Rohrmann等人，1979年）。本文给出了其他N端氨基酸序列。还通过使用固相放射免疫测定法对蛋白质进行抗原比较，研究了基于多角体蛋白分子进化确定的系统发育关系。结果表明，鳞翅目NPV与上述病毒组关系最为密切，与这些病毒的关系依次为：欧洲松叶蜂NPV、粉纹夜蛾颗粒体病毒和大蚊NPV。这些数据，结合杆状病毒的分布和有关昆虫系统发育的证据，表明杆状病毒与昆虫有着古老的联系，可能与它们一起进化。
