Sanfilippo J S, Barrows G H, Apkarian R P, Wittliff J L
Surg Gynecol Obstet. 1985 May;160(5):421-8.
A study using a Sprague-Dawley rat model was designed to correlate morphologic and intracellular steroid receptor alteration with danazol therapy. One uterine horn was removed for cytoplasmic estrogen receptor analysis following four to eight days of danazol therapy. This was followed by intra-aortic injection of buffered phosphate solution and 3 per cent glutaraldehyde. The remaining uterine horn was removed for scanning electron microscopic and morphometric analysis of light microscopic changes. After four days of treatment, there was an increase in the nuclear to cytoplasmic ratio compared with that of the control tissue. Vaginal smears on the eighth day of danazol exposure indicated that the rats had entered a noncycling estrus state, and there was a significant increase in the estrogen-specific binding capacity of the endometrial cells. Scanning electron microscopy showed a distinct decrease in endometrial folds, microvilli and glandular openings, which suggests a decline in secretory activity in the danazol-treated group. Thus, danazol induced measurable changes in the estrogen binding capacity of endometrial cells, and these changes correlated with certain morphologic alterations in the endometrial lining.
一项使用斯普拉格-道利大鼠模型的研究旨在将形态学和细胞内类固醇受体改变与达那唑治疗相关联。在达那唑治疗四至八天后，切除一个子宫角用于细胞质雌激素受体分析。随后进行主动脉内注射缓冲磷酸盐溶液和3%戊二醛。切除剩余的子宫角用于扫描电子显微镜检查和光镜变化的形态计量分析。治疗四天后，与对照组织相比，核质比增加。达那唑暴露第八天的阴道涂片表明大鼠已进入非周期性发情状态，并且子宫内膜细胞的雌激素特异性结合能力显著增加。扫描电子显微镜显示子宫内膜皱襞、微绒毛和腺开口明显减少，这表明达那唑治疗组的分泌活性下降。因此，达那唑诱导了子宫内膜细胞雌激素结合能力的可测量变化，并且这些变化与子宫内膜 lining 的某些形态学改变相关。
