Laurell C B, Rannevik G
Postgrad Med J. 1979;55 Suppl 5:40-3.
Analysis of 25 proteins of supposed hepatocyte origin was performed on plasma from 7 females before and during treatment with danazol. The same 25 proteins were analysed in plasma from pregnant women in their third trimester. The general conclusions are that the major plasma proteins of hepatic origin have a biosynthetic regulation which is steroid insensitive but the biosynthesis of some minor components is highly steroid sensitive in a way that supports the theory that hepatocyte steroid receptors exist. The full hepatocyte steroid response (2-6 weeks) develops more slowly than the response of steroid dependent tissues (days). The plasma protein response with danazol administration is similar to the response to other 17-alpha-alkylated anabolic steroids and is in accordance with the theory that danazol competes with endogenous steroids for their receptors in all target tissues. The changes during pregnancy are mainly referred to the increased influence of oestradiol. Integrated data from this and earlier studies suggest that the level of sex hormone binding globulin in a sensitive way reflects the competition of androgen, oestrogens and gestagens at hepatocyte receptor level.
对7名女性在服用达那唑治疗前及治疗期间的血浆进行了25种假定来源于肝细胞的蛋白质分析。对妊娠晚期孕妇的血浆也进行了同样的25种蛋白质分析。总的结论是，主要的肝脏来源血浆蛋白具有不依赖类固醇的生物合成调节，但一些次要成分的生物合成对类固醇高度敏感，这支持了肝细胞类固醇受体存在的理论。完整的肝细胞类固醇反应（2 - 6周）比类固醇依赖组织的反应（数天）发展得更慢。服用达那唑后的血浆蛋白反应与对其他17 - α - 烷基化合成代谢类固醇的反应相似，这与达那唑在所有靶组织中与内源性类固醇竞争其受体的理论相符。孕期的变化主要归因于雌二醇影响的增加。来自本研究及早期研究的综合数据表明，性激素结合球蛋白水平以一种敏感的方式反映了雄激素、雌激素和孕激素在肝细胞受体水平的竞争。
