Varga B, Horváth E, Folly G, Stark E
Biol Reprod. 1985 Apr;32(3):480-8. doi: 10.1095/biolreprod32.3.480.
CYF rats were anesthetized on various days of the 4-day cycle and blood samples were collected at 5-min interals from the ovarian vein before and after i.v. administration of 5 micrograms/100 g BW of luteinizing hormone (LH). Ovarian venous outflow, blood pressure and hematocrit were continuously recorded, and from the blood samples progesterone (P) and 17 beta-estradiol (E2) were determined by radioimmunoassay (RIA). Ovarian blood flow and P secretion showed a parallel increase on Day 1 (estrus), on Day 2, and on the afternoon of Day 4 (proestrous). LH increased ovarian blood flow each day of the cycle together with P and E2 secretion; however, no relationship was seen between the initial value of hormone secretion and the LH-induced increase of ovarian blood flow. Inhibition of hormone secretion by cycloheximide prevented the LH-induced increase of ovarian blood flow; moreover, a decrease in ovarian blood flow parallel with the diminution of hormone secretion was observed. Indomethacin pretreatment abolished the hyperemic effect of LH and partially inhibited the LH-induced increase of hormone secretion. Propranolol blocked the LH-induced increase of ovarian blood flow and blunted the effect of LH on hormone secretion. It was concluded that in LH-induced hyperemia, cAMP, prostaglandins and other vasoactive metabolites released during the process of hormone synthesis, and also a beta-adrenergic mechanism, are involved in the regulation of ovarian blood flow.
在4天周期的不同日子对CYF大鼠进行麻醉，在静脉注射5微克/100克体重的促黄体生成素（LH）之前和之后，每隔5分钟从卵巢静脉采集血样。连续记录卵巢静脉流出量、血压和血细胞比容，并通过放射免疫分析（RIA）测定血样中的孕酮（P）和17β-雌二醇（E2）。在第1天（发情期）、第2天和第4天下午（动情前期），卵巢血流量和P分泌呈平行增加。在整个周期的每一天，LH均增加卵巢血流量以及P和E2的分泌；然而，激素分泌的初始值与LH诱导的卵巢血流量增加之间未发现相关性。放线菌酮抑制激素分泌可阻止LH诱导的卵巢血流量增加；此外，观察到卵巢血流量的减少与激素分泌的减少平行。吲哚美辛预处理消除了LH的充血效应，并部分抑制了LH诱导的激素分泌增加。普萘洛尔阻断了LH诱导的卵巢血流量增加，并减弱了LH对激素分泌的作用。得出的结论是，在LH诱导的充血过程中，激素合成过程中释放的环磷酸腺苷（cAMP）、前列腺素和其他血管活性代谢产物，以及β-肾上腺素能机制，均参与卵巢血流量的调节。
