Paksy K, Varga B, Lázár P
National Institute of Occupational Health, Budapest, Hungary.
Biometals. 1992 Winter;5(4):245-50. doi: 10.1007/BF01061225.
Recently, cadmium has been described to disturb ovarian function in rats. In this paper the direct influence of cadmium on steroid production of ovarian cells in vitro has been studied. Granulosa and luteal cells were obtained from proestrous and pregnant rats, and incubated with 0, 5, 10, 20 or 40 micrograms ml-1 CdCl2 in the presence or absence of 0.1-1000 ng ml-1 follicle stimulating hormone (FSH) or luteinizing hormone (LH) for 24 or 48 h. Production of progesterone (P) and 17 beta-estradiol (E2) by granulosa and that of P by luteal cells were measured by radioimmunoassay. In FSH-stimulated granulosa cell cultures, 5 and 40 micrograms ml-1 CdCl2 suppressed P accumulation to 65 and 10%, respectively; accumulation of E2 (at 5 micrograms ml-1 CdCl2) decreased to 44%. P production of LH-supported luteal cells dropped to 86 and 66%, respectively, when 5 and 40 micrograms ml-1 CdCl2 was added to the medium. No alteration in basal P accumulation occurred in granulosa and luteal cell cultures following incubations with 20 and 40 micrograms ml-1 CdCl2, whereas basal E2 production of granulosa cells was markedly diminished. It is concluded that CdCl2 suppressing steroid synthesis in vitro exerts a direct influence on granulosa and luteal cell function.
最近，有研究表明镉会干扰大鼠的卵巢功能。本文研究了镉对体外培养的卵巢细胞甾体激素生成的直接影响。从动情前期和怀孕大鼠中获取颗粒细胞和黄体细胞，并在存在或不存在0.1 - 1000 ng/ml促卵泡激素（FSH）或促黄体生成素（LH）的情况下，用0、5、10、20或40微克/毫升的CdCl₂孵育24或48小时。通过放射免疫分析法测定颗粒细胞产生的孕酮（P）和17β - 雌二醇（E₂）以及黄体细胞产生的P。在FSH刺激的颗粒细胞培养物中，5和40微克/毫升的CdCl₂分别将P的积累抑制到65%和10%；E₂的积累（在5微克/毫升的CdCl₂时）降至44%。当向培养基中添加5和40微克/毫升的CdCl₂时，LH支持的黄体细胞的P产生分别降至86%和66%。用20和40微克/毫升的CdCl₂孵育后，颗粒细胞和黄体细胞培养物中的基础P积累没有变化，而颗粒细胞的基础E₂产生明显减少。结论是，CdCl₂在体外抑制甾体激素合成，对颗粒细胞和黄体细胞功能有直接影响。
