Morca Ali Ferhan, Coşkan Sevgi, Öncü Faruk, Akbaş Birol, Santosa Adyatma Irawan, Yeşil Serkan, Çelik Ali
Directorate of Plant Protection Central Research Institute, Gayret Mah. Fatih Sultan Mehmet Bulv, 06172, Yenimahalle, Ankara, Türkiye.
Department of Combating Forest Pests, General Directorate of Forestry, Beştepe Mah, 06560, Yenimahalle, Ankara, Türkiye.
Curr Microbiol. 2025 May 21;82(7):298. doi: 10.1007/s00284-025-04269-z.
Watermelon mosaic virus (WMV, genus Potyvirus) is a significant pathogen affecting cucurbit production in the Western Black Sea and Central Anatolia regions of Türkiye, with limited knowledge of its genetic diversity hindering molecular control strategies. In this study, 19 complete coat protein (CP) gene sequences from WMV isolates collected from four cucurbit species were analyzed using RT-PCR, MEGA11 for phylogenetics, and DnaSP v6.12.03 for population genetics. The phylogenetic analysis, including 48 global isolates, classified eight Turkish isolates into the "emerging" (EM) group, 11 into the "classic" (G1) group, and identified a novel minor phylogroup. While a recombination signal was detected in isolate KP164988, none was observed in the Turkish isolates. Genetic diversity analysis showed that the G1 group was less divergent than the EM group, with all populations under strong purifying selection (ω = 0.022-0.243) and displaying recent expansion, likely due to variations in newly reported isolates. The results emphasize the genetic separation between EM and G1 groups and reveal frequent gene flow between Turkish and global populations. This comprehensive survey provides valuable insights into the genetic structure and diversity of WMV in Türkiye, supporting other reports on minor phylogroups within the virus populations.
西瓜花叶病毒（WMV，马铃薯Y病毒属）是影响土耳其西部黑海地区和安纳托利亚中部地区葫芦科作物生产的一种重要病原体，对其遗传多样性了解有限阻碍了分子控制策略的实施。在本研究中，利用RT-PCR、MEGA11软件进行系统发育分析以及DnaSP v6.12.03软件进行群体遗传学分析，对从四种葫芦科作物中收集的19个WMV分离株的完整外壳蛋白（CP）基因序列进行了分析。系统发育分析涵盖48个全球分离株，将8个土耳其分离株归类为“新兴”（EM）组，11个归类为“经典”（G1）组，并鉴定出一个新的小分支。虽然在分离株KP164988中检测到重组信号，但在土耳其分离株中未观察到。遗传多样性分析表明，G1组的分歧程度低于EM组，所有群体均处于强烈的纯化选择之下（ω = 0.022 - 0.243）且呈现近期扩张趋势，这可能是由于新报道的分离株存在变异。研究结果强调了EM组和G1组之间的遗传分离，并揭示了土耳其和全球群体之间频繁的基因流动。这项全面的调查为土耳其WMV的遗传结构和多样性提供了有价值的见解，支持了关于该病毒群体内小分支的其他报道。
