Xu Yong, Chen Longzhi, Yang Liyan, Pan Lixia, Zheng Mengting, Shen Naikun, Yang Dengfeng
National Key Laboratory of Non-Food Biomass Energy Technology, Guangxi Academy of Sciences, Nanning, 530007, China.
Guangxi PFomic Information Technology Co., Ltd., Nanning, 530006, China.
Curr Microbiol. 2025 May 21;82(7):299. doi: 10.1007/s00284-025-04281-3.
A bacterial strain with chitin-degrading ability, designated FCG-7, was isolated from a mangrove sediment in Guangxi, PR China, and characterized using a polyphasic taxonomy approach. Cells of strain FCG-7 were Gram-stain-negative, aerobic, rod-shaped and formed milky-white colonies. Growth occurred at 15-37 °C (optimum, 30 °C), at pH 7-9 (optimum, pH 7-8) and with 0-1% NaCl (optimum, 0.5%). The major isoprenoid quinone was ubiquinone-8 (Q-8). The predominant fatty acids were summed feature 3 (C ω6c and/or Cω7c) and C. The polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol, five unidentified phospholipids and two unidentified aminophospholipids. The G + C content of the genomic DNA was 53.8%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain FCG-7 belonged to the genus Chitinibacter and was closely related to Chitinibacter bivalviorum 2T18 (similarity 99.29%) and Chitinibacter fontanus STM-7 (similarity 98.85%). The average nucleotide identity (ANI) values of FCG-7 and the above two type strains were 81.4-83.1%, and the digital DNA-DNA hybridization (dDDH) values were 22.9-24.3%. Based on the phylogenetic, phenotypic, chemotaxonomic and genotypic characteristics, strain FCG-7 represents a novel species of the genus Chitinibacter, for which the name Chitinibacter mangrovi sp. nov. is proposed. The type strain is FCG-7 (= KCTC 8742 = GDMCC 1.4868).
从中国广西的红树林沉积物中分离出一株具有几丁质降解能力的细菌菌株，命名为FCG-7，并采用多相分类法对其进行了特征描述。FCG-7菌株的细胞革兰氏染色阴性，好氧，杆状，形成乳白色菌落。在15-37℃（最适温度为30℃）、pH值7-9（最适pH值为7-8）和0-1%NaCl（最适浓度为0.5%）的条件下生长。主要类异戊二烯醌为泛醌-8（Q-8）。主要脂肪酸为总和特征3（C ω6c和/或Cω7c）和C。极性脂质包括磷脂酰乙醇胺、双磷脂酰甘油、五种未鉴定的磷脂和两种未鉴定的氨基磷脂。基因组DNA的G + C含量为53.8%。基于16S rRNA基因序列的系统发育分析表明，FCG-7菌株属于几丁质杆菌属，与双壳几丁质杆菌2T18（相似度99.29%）和泉几丁质杆菌STM-7（相似度98.85%）密切相关。FCG-7与上述两个模式菌株的平均核苷酸同一性（ANI）值为81.4-83.1%，数字DNA-DNA杂交（dDDH）值为22.9-24.3%。基于系统发育、表型、化学分类和基因型特征，FCG-7菌株代表了几丁质杆菌属的一个新物种，为此提出了新名称红树林几丁质杆菌（Chitinibacter mangrovi sp. nov.）。模式菌株为FCG-7（= KCTC 8742 = GDMCC 1.4868）。
