Division of Environmental Science & Ecological Engineering, College of Life Science & Biotechnology, Korea University, Seoul, Republic of Korea.
School of Earth and Environmental Science and Research Institute of Oceanography, Seoul National University, Seoul, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Dec;71(12). doi: 10.1099/ijsem.0.005134.
A yellow-coloured bacterium, designated strain JGD-16, was isolated from a tidal flat in Janggu-do, Garorim Bay, Taean-gun, Chungcheongbuk-do, Republic of Korea. Cells were Gram-stain-negative, aerobic, non-flagellated and short ovoid to coccoid-shaped. Growth was observed at 10-37 °C (optimum, 30 °C), pH 6.0-9.0 (pH 8.0) and with 1-5% (w/v) NaCl (2%). Results of 16S rRNA gene sequence analysis indicated that strain JGD-16 was closely related to LY02 (97.1 %), O30 (96.3 %), JPCCMB0017 (95.8 %), JCS350 (95.7 %) and BPTF-M16 (95.3%). Phylogenomic analysis using the maximum-likelihood algorithm showed that strain JGD-16 formed a clade with the genus . The genomic DNA G+C content was 57.8 mol%. The predominant respiratory quinone was ubiquinone-10. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, a sphingoglycolipid, an unidentified glycolipid and an unidentified lipid. The major fatty acids were C 7 (31.5 %) and C 6 (19.6 %). On the basis of its phylogenomic, physiological and chemotaxonomical characteristics, strain JGD-16 represents a novel species within the genus , for which the name JGD-16sp. nov. is proposed. The type strain is JGD-16 (=KCTC 72632=KACC 21405=JCM 33750). We also propose the reclassification of as comb. nov., as comb. nov. and as comb. nov.
一株黄色细菌，命名为 JGD-16 菌株，从韩国忠清北道泰安郡牙山湾加罗林湾江居岛的潮滩中分离得到。细胞革兰氏染色阴性，需氧，无鞭毛，呈短卵圆形至球形。在 10-37°C（最佳温度为 30°C）、pH 值 6.0-9.0（pH 值 8.0）和 1-5%（w/v）NaCl（2%）的条件下可以生长。16S rRNA 基因序列分析结果表明，菌株 JGD-16 与 LY02（97.1%）、O30（96.3%）、JPCCMB0017（95.8%）、JCS350（95.7%）和 BPTF-M16（95.3%）最为相近。基于最大似然算法的系统发育基因组分析表明，菌株 JGD-16 与属形成一个分支。基因组 DNA G+C 含量为 57.8mol%。主要呼吸醌为泛醌-10。主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱、神经酰胺糖脂、一种未鉴定的糖脂和一种未鉴定的脂类。主要脂肪酸为 C7（31.5%）和 C6（19.6%）。基于其系统发育、生理和化学分类特征，菌株 JGD-16 代表属内的一个新种，因此命名为 JGD-16sp. nov.。模式菌株为 JGD-16（=KCTC 72632=KACC 21405=JCM 33750）。我们还提议将重新分类为 ， 为 ， 为 。
