Smirnova Ekaterina I, Shestakova Victoria A, Isaeva Elena V, Kisel Anastas A, Yatsenko Elena M, Shegay Peter V, Kaprin Andrey D, Klabukov Ilya D, Baranovskii Denis S
National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Obninsk, Russia.
Methods Mol Biol. 2025;2939:281-289. doi: 10.1007/7651_2025_632.
Chondrocyte-based strategies are widely used for cartilage repair and show promising results in the treatment of articular cartilage damage and osteoarthritis. In cartilage repair approaches that involve the implantation of autologous chondrocytes or tissue-engineered scaffolds, it is essential to obtain significant amounts of viable cells. Nasal chondrocytes serve as a clinically valuable source of cells for cartilage regeneration, and their isolation is a critical step in cartilage tissue engineering. This chapter outlines an optimized protocol for obtaining nasal chondrocytes from neonatal rats using a Leica VT1200 vibratome to obtain nasal cartilage fragments. Enzymatic isolation of nasal chondrocytes and their subsequent in vitro culture highlight the importance of the stem and progenitor cell niche in supporting the further culture and differentiation of these cells for effective cartilage regeneration.
基于软骨细胞的策略被广泛用于软骨修复，并在治疗关节软骨损伤和骨关节炎方面显示出有前景的结果。在涉及植入自体软骨细胞或组织工程支架的软骨修复方法中，获得大量活细胞至关重要。鼻软骨细胞是软骨再生临床上有价值的细胞来源，其分离是软骨组织工程中的关键步骤。本章概述了一种优化方案，该方案使用徕卡VT1200振动切片机从新生大鼠获取鼻软骨碎片，从而获得鼻软骨细胞。鼻软骨细胞的酶解分离及其随后的体外培养突出了干细胞和祖细胞生态位在支持这些细胞进一步培养和分化以实现有效软骨再生方面的重要性。
