Wang 王晨 Chen, Zhao 赵洪 Hong, Zhang 张洪魁 Hongkui, Sun 孙思杰 Sijie, Xue 薛勇彪 Yongbiao
National Genomics Data Center, China National Center for Bioinformation, Beijing 100101, China.
Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
Genomics Proteomics Bioinformatics. 2025 Jul 11;23(3). doi: 10.1093/gpbjnl/qzaf046.
Self-incompatibility (SI) is an important genetic mechanism in angiosperms that prevents inbreeding and promotes outcrossing, with significant implications for crop breeding, including genetic diversity, hybrid seed production, and yield optimization. In eudicots, SI is typically governed by a single S-locus containing tightly linked pistil and pollen S-determinant genes. Despite major advances in SI research, a centralized, comprehensive resource for SI-related genomic data remains lacking. To address this gap, we developed the Plant Self-Incompatibility Atlas (PSIA), a systematically curated knowledgebase providing an extensive compilation of plant SI, including genomic resources for SI species, S gene annotations, molecular mechanisms, phylogenetic relationships, and comparative genomic analyses. The current release of PSIA includes over 500 genome assemblies from 469 SI species. Using known S genes as queries, we manually identified and rigorously curated 3700 S genes. PSIA provides detailed S-locus information from assembled genomes of SI species and offers an interactive platform for browsing, BLAST searches, S gene analysis, and data retrieval. Additionally, PSIA serves as a unique platform for comparative genomic studies of S-loci, facilitating exploration of the dynamic processes underlying the origin, loss, and regain of SI. As a comprehensive and user-friendly resource, PSIA will greatly advance our understanding of angiosperm SI and serve as a valuable tool for crop breeding and hybrid seed production. PSIA is freely available at http://www.plantsi.cn.
自交不亲和性（SI）是被子植物中一种重要的遗传机制，可防止近亲繁殖并促进异花授粉，对作物育种具有重要意义，包括遗传多样性、杂交种子生产和产量优化。在双子叶植物中，SI通常由一个单一的S位点控制，该位点包含紧密连锁的雌蕊和花粉S决定基因。尽管SI研究取得了重大进展，但仍缺乏一个集中的、全面的SI相关基因组数据资源。为了填补这一空白，我们开发了植物自交不亲和性图谱（PSIA），这是一个经过系统整理的知识库，提供了植物SI的广泛汇编，包括SI物种的基因组资源、S基因注释、分子机制、系统发育关系和比较基因组分析。PSIA的当前版本包括来自469个SI物种的500多个基因组组装。以已知的S基因为查询对象，我们手动识别并严格整理了3700个S基因。PSIA提供了来自SI物种组装基因组的详细S位点信息，并提供了一个交互式平台，用于浏览、BLAST搜索、S基因分析和数据检索。此外，PSIA作为S位点比较基因组研究的独特平台，有助于探索SI起源、丧失和恢复的动态过程。作为一个全面且用户友好的资源，PSIA将极大地促进我们对被子植物SI的理解，并作为作物育种和杂交种子生产的宝贵工具。PSIA可在http://www.plantsi.cn免费获取。
