Liu Ziyu, Ruan Shuchao, Wang Junhui, Li Weiyan, Tian Ye, Zhao Chong, Han Lingshu, Wang Luo, Chang Yaqing, Ding Jun
Liaoning Provincial Key Laboratory of Northern Aquatic Germplasm Resources and Genetics and Breeding, Dalian Ocean University, Dalian, Liaoning 116023, PR China; Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, Liaoning 116023, PR China.
Liaoning Provincial Key Laboratory of Northern Aquatic Germplasm Resources and Genetics and Breeding, Dalian Ocean University, Dalian, Liaoning 116023, PR China; Aquatic Germplasm Resources Conservation and Utilization Engineering Research Center in Liaoning Province, Dalian Ocean University, Dalian, Liaoning 116023, PR China; Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, Liaoning 116023, PR China.
Comp Biochem Physiol Part D Genomics Proteomics. 2025 May 14;56:101536. doi: 10.1016/j.cbd.2025.101536.
This study primarily investigated the light spectra that increased the number of papillae in sea cucumbers and the molecular pathways that contributed to the growth of papillae under these light conditions. At 8.0-15.0 °C, sea cucumbers were subjected to a 60-day indoor experiment under different lighting environments (normal, dark, blue light, yellow light, green light, red light, and white light). The number of papillae was analyzed using repeated measures ANOVA, and transcriptome sequencing data of the papillae were subsequently analyzed. The analysis revealed that there were significant differences in the number of papillae within each group at the beginning of the experiment and on days 15, 30, and 45 of light exposure (p < 0.05), while no significant differences were observed within each group between days 45 and 60 of light exposure (p > 0.05). Under blue light exposure, the number of papillae and the increase in the number of papillae in sea cucumbers showed significant differences compared to the control group (p < 0.05). The pathways related to the increase in the number of papillae in sea cucumbers include phenylalanine, tyrosine, and tryptophan biosynthesis and phenylalanine metabolism. The number of papillae increased the most after 45 days of blue light exposure, after which it stabilized. It was speculated that the increase in the number of papillae might be related to amino acid metabolism and collagen synthesis.
本研究主要调查了能增加海参乳头数量的光谱以及在这些光照条件下促进乳头生长的分子途径。在8.0 - 15.0°C的温度下，将海参置于不同光照环境（正常、黑暗、蓝光、黄光、绿光、红光和白光）中进行为期60天的室内实验。使用重复测量方差分析对乳头数量进行分析，随后对乳头的转录组测序数据进行分析。分析结果显示，在实验开始时以及光照暴露的第15、30和45天，每组内的乳头数量存在显著差异（p < 0.05），而在光照暴露的第45天至60天之间，每组内未观察到显著差异（p > 0.05）。在蓝光照射下，海参的乳头数量以及乳头数量的增加与对照组相比显示出显著差异（p < 0.05）。与海参乳头数量增加相关的途径包括苯丙氨酸、酪氨酸和色氨酸生物合成以及苯丙氨酸代谢。蓝光照射45天后乳头数量增加最多，之后趋于稳定。推测乳头数量的增加可能与氨基酸代谢和胶原蛋白合成有关。
