García-Sánz J A, Ruiz-Puente J, Jiménez-Paredes J, González-Pacheco M, Villalva-Posada H
Vaccine. 1985 Mar;3(1):23-6. doi: 10.1016/0264-410x(85)90005-2.
The biological activities of whole-cell pertussis vaccine and a new acellular preparation were compared. It was observed that both preparations were similarly effective in inducing protection in mice against intracerebral challenge with B. pertussis, however, the acellular preparation was approximately 200-fold more potent. A close relationship between the degree of pertussis toxin purification and the increase in potency was observed. Neither preparation affected the weight gain of the animals within the doses employed. As expected, both preparations had histamine-sensitizing activity and leukocyte-promoting activity. It is suggested that this preparation, obtained using very simple methodology, can be considered as a potentially useful acellular pertussis vaccine.
对全细胞百日咳疫苗和一种新的无细胞制剂的生物活性进行了比较。观察到两种制剂在诱导小鼠抵抗百日咳博德特氏菌脑内攻击的保护作用方面同样有效，然而，无细胞制剂的效力大约高200倍。观察到百日咳毒素纯化程度与效力增加之间存在密切关系。在所使用的剂量范围内，两种制剂均未影响动物的体重增加。正如预期的那样，两种制剂都具有组胺致敏活性和促白细胞活性。有人提出，使用非常简单的方法获得的这种制剂可被视为一种潜在有用的无细胞百日咳疫苗。
