Saran G
J Biol Stand. 1985 Oct;13(4):315-20. doi: 10.1016/s0092-1157(85)80045-7.
A vaccine was prepared from the growth of Bordetella pertussis by repeated treatment with acetone. The vaccine has been designated as acetone-treated pertussis vaccine (ATPV). A total of ten batches of ATPV were prepared, five each from B. pertussis strains 134 and 509. These strains are routinely employed at this Institute for the production of conventional whole-cell pertussis vaccine (WCPV) for blending in diphtheria-pertussis-tetanus vaccine. The mouse protective and histamine sensitizing activities of ATPV and WCPV were compared. The ATPV showed 1.5- to 2-fold higher potency than the WCPV. The histamine sensitizing activity of ATPV was much reduced compared with that of the WCPV. No appreciable difference was observed in the results of a mouse weight-gain tests between the ATPV and WCPV. The details of the preparation of ATPV have been described. Because of higher potency and reduced histamine sensitizing activity, the ATPV may prove more acceptable in immunization programmes against pertussis, even in countries where WCPV is unpopular due to its suspected reactogenicity.
通过用丙酮反复处理百日咳博德特氏菌的培养物制备了一种疫苗。该疫苗被指定为丙酮处理的百日咳疫苗（ATPV）。共制备了十批ATPV，其中五批来自百日咳博德特氏菌菌株134，五批来自菌株509。该研究所通常使用这些菌株生产用于白喉-百日咳-破伤风疫苗混合的常规全细胞百日咳疫苗（WCPV）。比较了ATPV和WCPV的小鼠保护活性和组胺致敏活性。ATPV的效力比WCPV高1.5至2倍。与WCPV相比，ATPV的组胺致敏活性大大降低。在ATPV和WCPV之间的小鼠体重增加试验结果中未观察到明显差异。已经描述了ATPV的制备细节。由于效力更高且组胺致敏活性降低，即使在因怀疑有反应原性而WCPV不受欢迎的国家，ATPV在百日咳免疫计划中可能也会被证明更易接受。
