Elshafie Elshafie I, Al-Habsi Khalid, Ali Haytham, ElTahir Yasmin, Al-Kharousi Kaadhia, Al-Hamrashdi Abeer, Khojali Mohamed, Al-Batrani Ali M, Johnson Eugene H
Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Oman; Central Veterinary Research Laboratory, Al Amarat, Khartoum, Sudan.
Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Oman.
Vet Parasitol Reg Stud Reports. 2025 Jun;61:101273. doi: 10.1016/j.vprsr.2025.101273. Epub 2025 May 6.
The Omani market imports live sheep and goats from across the globe to meet the high human demand for fresh meat. However, this reliance on imports may increase the risk of introducing foreign pathogens despite the strict quarantine regulations. Therefore, we aimed to determine endoparasites among imported sheep and goats that may escape through the sole quarantine station in Muscat. For this purpose, 205 sheep and 47 goats' fecal samples were collected from animals imported from different countries at the Central Quarantine Station in Muscat between 2019 and 2020. The identification and quantification of parasites were revealed by parasitological and molecular techniques, and sequencing analyses were performed on the highly prevalent parasites. The fecal flotation test showed an overall prevalence of gastrointestinal parasites in sheep and goats at 73.17 % and 78.72 %, respectively. The most abundant enteric parasites were Eimeria spp. (65.08 %), strongyle nematodes (25.7 %), and mixed infections of both (19.1 %). In contrast, Strongyloides spp. (2.78 %), Trichuris spp. (2.38 %), and Moniezia spp. (0.40 %) were present to a lesser extent. The McMaster technique revealed an average of 15,325 oocysts and 646 eggs per gram of feces for Eimeria spp. and strongyle-type eggs, respectively. The sequence analyses of the infected sheep and goats showed high similarity with sequences of Trichostrongylus spp., Oesophagostomum columbianum, Haemonchus contortus, and Eimeria spp. Rigorous control measures are essential at Omani quarantine stations to inhibit the entry of new parasites into the country and to mitigate the dissemination of various cross-border gastrointestinal parasites. This may pose a significant risk to public health and livestock productivity.
阿曼市场从全球进口活羊和山羊，以满足人类对新鲜肉类的高需求。然而，尽管有严格的检疫规定，但这种对进口的依赖可能会增加引入外来病原体的风险。因此，我们旨在确定进口绵羊和山羊体内可能通过马斯喀特唯一的检疫站逃逸的体内寄生虫。为此，2019年至2020年期间，在马斯喀特的中央检疫站从不同国家进口的动物中采集了205份绵羊和47份山羊的粪便样本。通过寄生虫学和分子技术对寄生虫进行鉴定和定量，并对高度流行的寄生虫进行测序分析。粪便漂浮试验显示，绵羊和山羊胃肠道寄生虫的总体患病率分别为73.17%和78.72%。最常见的肠道寄生虫是艾美耳属（65.08%）、圆线虫（25.7%）以及两者的混合感染（19.1%）。相比之下，类圆线虫属（2.78%）、毛首线虫属（2.38%）和莫尼茨绦虫属（0.40%）的感染程度较低。麦克马斯特技术显示，每克粪便中艾美耳属卵囊和圆线虫型虫卵的平均数量分别为15325个和646个。对感染绵羊和山羊的测序分析表明，其与毛圆线虫属、哥伦比亚食道口线虫、捻转血矛线虫和艾美耳属的序列高度相似。阿曼检疫站必须采取严格的控制措施，以阻止新的寄生虫进入该国，并减轻各种跨境胃肠道寄生虫的传播。这可能对公众健康和牲畜生产力构成重大风险。
