Larrea F, Villanueva C, Carmen Cravioto M, Escorza A, del Real O
Fertil Steril. 1985 Jul;44(1):25-30.
The heterogeneity of human serum prolactin (PRL) in 12 women with hyperprolactinemia and different derangements of the hypothalamic-pituitary-ovarian axis was studied. The patients were subdivided into three groups: four women with hyperprolactinemia and normal ovarian function (group I), four women with hyperprolactinemia associated with sporadic endometrial bleedings and a positive progestin test (group II), and four women with amenorrhea and a negative progestin test (group III). Gel filtration chromatography of serum samples from patients in group I revealed in three of them that most (80%) of their immunoreactive PRL eluted as big,big PRL (Mr greater than 100,000); whereas the rest of the patients, including those from groups II and III, exhibited a distribution pattern similar to that obtained in normal menstruating women. All PRL species had similar affinities for the antibody, as disclosed by the slopes generated in dose-response curves. These results indicate that ovarian function in hyperprolactinemia might be dependent upon PRL heterogeneity and suggest that big,big PRL may have, under in vivo conditions, a low degree of biologic activity.
对12名高催乳素血症且下丘脑 - 垂体 - 卵巢轴有不同紊乱情况的女性的人血清催乳素（PRL）异质性进行了研究。患者被分为三组：4名高催乳素血症且卵巢功能正常的女性（第一组），4名高催乳素血症伴散发性子宫内膜出血且孕激素试验阳性的女性（第二组），以及4名闭经且孕激素试验阴性的女性（第三组）。对第一组患者的血清样本进行凝胶过滤层析发现，其中3名患者大部分（80%）的免疫反应性PRL以大大PRL（Mr大于100,000）的形式洗脱；而其余患者，包括第二组和第三组的患者，其分布模式与正常月经周期女性相似。剂量反应曲线产生的斜率表明，所有PRL种类对抗体的亲和力相似。这些结果表明，高催乳素血症中的卵巢功能可能取决于PRL的异质性，并提示在体内条件下，大大PRL可能具有较低程度的生物活性。
