Emergence and transmission of Carbapenem-resistant gene in healthy pigs in Baise, Guangxi, China: a discovery.

A total of 366 tonsillar tissue samples were collected from healthy free-range pigs owned by farmers across 12 districts in Baise City, Guangxi, China. This initiative successfully isolated six strains of Carbapenem-Resistant (CRE), including four strains of , and one of and . Assessments utilizing Carbapenemase inhibitor enhancement test, in conjunction with the ResFinder resistance gene database, revealed that five strains carried the gene, while one strain possessed the gene. The overall positivity rate for CRE was determined to be 1.64%. Conjugation tests further demonstrated the ability of the genes to transfer to recipient strains. Additionally, an analysis of the genetic environment surrounding the genes showed similarities among the genes within the same geographic region, suggesting that these strains may have originated from a common ancestor and spread horizontally among different clones. This study provides valuable insights into the transmission of CRE among free-range farmers in Baise City, Guangxi, China.