Ma Fang, Wang Wenli, Dong Jiaxuan, Zhou Xiangjun, Lin Zhiyun, Zheng Pan, Nian Xiajiao, Dong Lili
School of Bioengineering and Technology, Tianshui Normal University, South Xihe Road, Qinzhou District, Tianshui, Gansu Province, 741000, P. R. China.
Dingxi Vocational and Technical College, Dingxi, 730500, China.
Mol Biol Rep. 2025 May 22;52(1):486. doi: 10.1007/s11033-025-10601-4.
To investigate the response of the suppressor of the cytokine signaling (socs) gene family in rainbow trout following exposure to microplastics, this study conducted a bioinformatics analysis of the socs gene family using rainbow trout genome data, complemented by experiments involving microplastic exposure and gene expression detection.
The findings revealed that the rainbow trout SOCS gene family comprises 27 members, encoding proteins with lengths ranging from 110 to 837 amino acids. Analyses of motifs, domains, and gene structures indicate that members of this family are highly conserved. RNA sequencing data demonstrated that, following microplastic exposure, the expression levels of socs1, socs2, socs3, socs5, socs6, socs7, and cish in the liver, intestine, and brain tissues of rainbow trout underwent significant changes. Additionally, RT-qPCR results indicated that the expression levels of several socs genes were down-regulated, whereas socs1a, socs1b, socs7a1, socs7b1, and socs7b2 exhibited significant up-regulation. These genes may play crucial roles in the response to microplastic exposure in rainbow trout.
This study elucidates the involvement of the socs gene family members in the context of microplastic exposure, providing valuable insights into the underlying toxicological mechanisms and enhancing our understanding of the threats posed by plastic pollution to freshwater organisms.
为了研究虹鳟鱼在暴露于微塑料后细胞因子信号传导抑制因子（socs）基因家族的反应，本研究利用虹鳟鱼基因组数据对socs基因家族进行了生物信息学分析，并辅以微塑料暴露和基因表达检测实验。
研究结果显示，虹鳟鱼SOCS基因家族由27个成员组成，编码的蛋白质长度在110至837个氨基酸之间。对基序、结构域和基因结构的分析表明，该家族成员具有高度保守性。RNA测序数据表明，在暴露于微塑料后，虹鳟鱼肝、肠和脑组织中socs1、socs2、socs3、socs5、socs6、socs7和cish的表达水平发生了显著变化。此外，RT-qPCR结果表明，几个socs基因的表达水平下调，而socs1a、socs1b、socs7a1、socs7b1和socs7b2则表现出显著上调。这些基因可能在虹鳟鱼对微塑料暴露的反应中发挥关键作用。
本研究阐明了socs基因家族成员在微塑料暴露情况下的参与情况，为潜在的毒理学机制提供了有价值的见解，并增强了我们对塑料污染对淡水生物构成的威胁的理解。
