Preston C M, Jenkins T
Z Parasitenkd. 1985;71(3):373-81. doi: 10.1007/BF00928339.
The structure and development of the polar plugs of Trichuris muris eggs were observed by light and electron microscopy. Initial stages in plug formation commenced in the spermatheca of the adult female where two polar papillae became delimited from the remainder of the oocyte cytoplasm. These papillae exhibited a discrete PAS positive reaction and were regarded as prospective plug regions from which cytoplasmic granules were absent. Glycogen rosettes, initially concentrated in these prospective areas, were later transformed into an irregular fine network of chitin-protein microfibrils. This arrangement of microfibrils was in contrast to that of the surrounding collar region which displayed a distinct lamellate organization. The fully developed polar plug was of lower electron density than the shell. Each plug was covered externally by the vitelline layer and lined internally by the lipid layer of the eggshell.
利用光学显微镜和电子显微镜观察了鼠鞭虫卵极栓的结构和发育过程。极栓形成的初始阶段始于成年雌虫的受精囊，在那里两个极乳头从卵母细胞的其余细胞质中分隔出来。这些乳头呈现出离散的过碘酸希夫（PAS）阳性反应，被视为未来的栓区域，其中没有细胞质颗粒。糖原玫瑰花结最初集中在这些预期区域，后来转化为几丁质 - 蛋白质微纤维的不规则精细网络。这种微纤维的排列与周围呈现明显层状组织的领区不同。完全发育的极栓的电子密度低于卵壳。每个栓外部被卵黄膜覆盖，内部由蛋壳的脂质层衬里。
