Kiarie Elijah G, Cheng Veronica, Njeri Felix M, Bakhshalinejad Reza, Amerah Ahmed M, Fickler Anna, Sokale Adebayo O
Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
Poult Sci. 2025 May 10;104(8):105285. doi: 10.1016/j.psj.2025.105285.
The impact of supplementing multiple doses of single ß-mannanase (BM) in corn and soybean meal-based diets was investigated in Lohmann LSL lite hens. Corn and soybean meal-based diets were formulated to meet or exceed nutrient specifications and supplemented with BM at 0, 200, 400, 800 and 1,200 TMU/kg of feed. All diets contained titanium dioxide indigestible marker and were prepared in mash form. Three hundred and twenty hens at 63 weeks of age (woa) were placed in enriched cages (4 birds/cage). Diets were allocated to give 16 replicate cages/diet. Birds had free access to water and feed to 83 woa. Hen day egg production (HDEP), feed intake (FI), FCR, egg weight (EW), egg mass (EM), eggshell breaking strength (ESBS) and thickness (EST), Haugh unit (HU) and yolk color were reported at 4 weeks periods. Apparent retention (AR) of components and metabolizable energy (AME) were determined at 4th and 17th week of the experiment. Plasma metabolites, ceca digesta short chain fatty acids (SCFA), body and organ weight were determined at the end of the experiment. Interactions (P ≤ 0.05) between BM and period/sampling time point was observed for HDEP, EW, EM, FCR and AR of neutral detergent fiber (ARNDF) such that there were period dependent linear and non-linear responses to BM. Independent of the period, BM increased HDEP (P = 0.025, cubically), EW (P ≤ 0.018, linearly and quartically), EM (P < 0.01, quartically). The FCR reduced linearly (P = 0.032) in response to BM. Birds receiving BM800 had higher ARNDF than birds receiving BM0 at 4th wk sampling. The ESBS increased (P = 0.040, cubically) whereas HU decreased (P = 0.048, quadratically) in response to BM. The AR of DM, CP and AME, plasma glucose, cholesterol, lactate dehydrogenase, lipase Ca, P, and abdominal fat increased (P < 0.05) linearly in response to BM. There were no BM effects (P > 0.05) on SCFA. In conclusion, BM improved HDEP; egg quality and nutrient retention dose dependently. Moreover, plasma metabolites indicated positive effects on indices of carbohydrate and lipid metabolism.
在罗曼LSL lite蛋鸡中研究了在以玉米和豆粕为基础的日粮中添加多剂量单一β-甘露聚糖酶（BM）的影响。以玉米和豆粕为基础的日粮按满足或超过营养标准进行配制，并分别添加0、200、400、800和1200 TMU/kg饲料的BM。所有日粮均含有二氧化钛这种不可消化标记物，并制成粉料形式。将320只63周龄的母鸡放入改良鸡笼（每笼4只鸡）。日粮分配方式为每种日粮设16个重复鸡笼。母鸡可自由饮水和采食至83周龄。每隔4周记录母鸡日产蛋量（HDEP）、采食量（FI）、料蛋比（FCR）、蛋重（EW）、蛋重产量（EM）、蛋壳破裂强度（ESBS）和厚度（EST）、哈氏单位（HU）以及蛋黄颜色。在试验的第4周和第17周测定各成分的表观存留率（AR）和代谢能（AME）。在试验结束时测定血浆代谢物、盲肠内容物短链脂肪酸（SCFA）以及体重和器官重量。观察到BM与时期/采样时间点之间对HDEP、EW、EM、FCR和中性洗涤纤维的表观存留率（ARNDF）存在交互作用（P≤0.），即对BM存在时期依赖性线性和非线性反应。不考虑时期因素，BM提高了HDEP（P = 0.025，呈三次方关系）、EW（P≤0.018，呈线性和四次方关系）、EM（P<0.01，呈四次方关系）。FCR随BM呈线性降低（P = 0.032）。在第4周采样时，接受BM800的鸡的ARNDF高于接受BM0的鸡。ESBS随BM增加（P = 0.040，呈三次方关系），而HU随BM降低（P = 0.048，呈二次方关系）。DM、CP和AME的AR、血浆葡萄糖、胆固醇、乳酸脱氢酶、脂肪酶、钙、磷以及腹脂随BM呈线性增加（P<0.05）。BM对SCFA没有影响（P>0.05）。总之，BM可提高HDEP；对蛋品质和养分存留呈剂量依赖性。此外，血浆代谢物表明对碳水化合物和脂质代谢指标有积极影响。
